Aim: To determine the expression patterns of the spliced variants during arsenic trioxide-mediated cell cycle arrest and curcumin-induced apoptosis in MCF-7 cells.
Materials & Methods: AsO and curcumin were used to study cytotoxicity, cell cycle arrest, apoptosis and the expression of variants. The MUSE Cell Analyser was used to analyze cell cycle arrest, apoptosis and multicaspase activity while apoptosis was further confirmed using microscopy. Semi-quantitative RT-PCR was employed to quantitate the expression of the variants.
Results: This study showed that the MCF-7 cells expressed variant 1 but lacked both variant 2 and variant 3. Both AsO and curcumin significantly downregulated variant 1 (p < 0.001).
Conclusion: variants are promising therapeutic targets.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6745614 | PMC |
| http://dx.doi.org/10.2144/fsoa-2019-0047 | DOI Listing |
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