AI Article Synopsis
- A significant percentage (3% to 28%) of individuals infected with the chikungunya virus (CHIKV) show no symptoms, highlighting the need for better screening methods beyond traditional diagnostic tests like ELISAs.* -
- This study developed two new monoclonal antibodies targeting CHIKV's E1 protein and utilized them in a fluorescence-linked immunosorbent assay (FLISA), which proved to be more effective than ELISA in detecting the virus.* -
- FLISA demonstrated a lower limit of detection, allowing it to identify CHIKV more accurately in human samples, outperforming ELISA, especially in the presence of human sera and blood.*
Article Abstract
Background: When infected with the chikungunya virus (CHIKV), 3% to 28% of CHIKV-infected individuals remain asymptomatic, necessitating the development of improved high-throughput screening methods to overcome the limitations of molecular diagnostics or enzyme-linked immunosorbent assays (ELISAs).
Objective: In this study, two novel monoclonal antibodies (mAbs) targeting envelope 1 (E1) of CHIKV were developed and applied in a fluorescence-linked immunosorbent assay (FLISA) using coumarin-derived dendrimer as the fluorophore.
Methods: The performance of the FLISA was compared with that of ELISA.
Results: Using the two novel mAbs (2B5 and 2C8), FLISA could detect 1 × 105 PFU/mL of CHIKV, exhibiting a 2-fold lower limit of detection (LOD) compared to ELISA. The LOD of FICT corresponded to a comparative threshold value of 23.95 and 4 × 106 of RNA copy number/µL. In the presence of human sera and blood, virus detection by FLISA was 3-fold better than ELISA, with an LOD of 2 × 105 PFU/mL. Sera and blood interfered with the ELISA, resulting in 6 × 105 PFU/mL as the LOD.
Conclusions: FLISA using two novel mAbs and coumarin-derived dendrimer is a superior diagnostic assay for detecting CHIKV in human sera and blood, compared to conventional ELISA.
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| http://dx.doi.org/10.1159/000502823 | DOI Listing |
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