is a valuable plant for both ornament and flower border. As a major constraint, low temperature affects the growing development and survival of . However, there are few systematic studies in terms of molecular mechanism on the tolerance of low temperature in . In this study, Illumina sequencing technology was applied to analyze the cold resistance mechanism of plants. Six cDNA libraries were obtained from two samples of two groups, the cold-treated group and the control group. A total of 271,920 unigenes were produced from 406,641 assembled transcripts. Among these, 19,003 differentially expressed genes (DEGs) (corrected -value <0.01, |log2(fold change) | >3) were obtained, including 9852 upregulated and 9151 downregulated genes. The antioxidant enzyme system, photosynthesis, plant hormone signal transduction, fatty acid metabolism, starch and sucrose metabolism pathway, and transcription factors were analyzed. Based on these results, series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to response mechanism of low temperature were tested. Eleven upregulated DEGs were validated by Quantitative Real-time PCR. In this study, we provided the transcriptome sequence resource of and used these data to realize its molecular mechanism under cold stress. The results contributed to valuable clues for genetic studies and helped to screen candidate genes for cold-resistance breeding.
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http://dx.doi.org/10.1089/dna.2019.4707 | DOI Listing |
Vision Res
January 2025
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Elevated intraocular pressure (IOP) is a significant risk factor for glaucoma, causing structural and functional damage to the eye. Increased IOP compromises the metabolic and structural integrity of retinal ganglion cell (RGC) axons, leading to progressive degeneration and influencing the ocular immune response. This study investigated early cellular and molecular changes in the retina and optic nerve (ON) following ocular hypertension (OHT).
View Article and Find Full Text PDFACS Appl Mater Interfaces
January 2025
International Science and Technology Cooperation Base of Energy Materials and Application, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, P.R. China.
Poly(ethylene oxide) (PEO) has been widely studied as an electrolyte owing to its excellent lithium compatibility and good film-forming properties. However, its electrochemical performance at room temperature remains a significant challenge due to its low ionic conductivity, narrow electrochemical window, and continuous decomposition. Herein, we prepare a multifunctional polar polymer to optimize PEO's electrochemical properties and cycling stability.
View Article and Find Full Text PDFVet Res Commun
January 2025
Faculty of Agriculture, University Farm, Utsunomiya University, Tochigi, 321-4415, Japan.
The purpose of this study was to improve the quality of frozen-thawed canine spermatozoa through the optimization of glycerol concentration (GC) and freezing rate in the semen freezing protocol. Ejaculates from nine dogs were diluted with an extender containing 0%, 1.5%, 3%, 6%, or 9% glycerol.
View Article and Find Full Text PDFChem Commun (Camb)
January 2025
University of Strasbourg Institute for Advanced Study (USIAS), Strasbourg, France.
A series of four original phosphine-free thioether-NHC manganese complexes have been synthesised and fully characterized. These complexes have been applied as efficient catalysts for the hydrogenation of alkenes and ketones at room temperature, with low catalyst loadings (TON up to 900).
View Article and Find Full Text PDFACS Appl Bio Mater
January 2025
Department of Chemistry, University of North Dakota, Grand Forks, North Dakota 58202, United States.
Synergistic photodynamic/photothermal therapy (PDT/PTT) can be used to target cancer cells by locally generating singlet oxygen species or increasing temperature under laser irradiation. This approach offers higher tumor ablation efficiency, lower therapeutic dose requirements, and reduced side effects compared to single treatment approaches. However, the therapeutic efficiency of PDT/PTT is still limited by the low oxygen levels within the solid tumors caused by abnormal vasculature and altered cancer cell metabolism.
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