[Effects of freeze-thaw and soil moisture on content and spectral structure properties of dissolved organic matter in forest soil leachates.].

The contents and stability of soil dissolved organic matter (DOM) can affect key processes of soil carbon and nitrogen cycle. The responses of DOM content and its spectral structure pro-perties in forest soils to climate change remain unclear. We collected soil samples from two temperate forests, i.e., the broadleaf and Korean pine mixed forest (BKPF) and adjacent secondary white birch forest (WBF), in Changbai Mountains, northeastern China. Using a combination of three-dimensional fluorescence spectrum and parallel factor analysis, a simulated freeze-thaw experiment was conducted in the laboratory. We examined the effects of freeze-thaw intensity, freeze-thaw cycle and their interaction on the content, components and spectral properties of DOM leached from the two forest surface soils with different moisture levels. The results showed that DOM content and components of soil leachates varied with forest types, soil moisture, freeze-thaw intensity and freeze-thaw cycle. The DOM content in the leachates was lowest at medium moisture level and was significantly affected by the high freeze-thaw intensity. In addition, the DOM content increased first and then decreased with the increases of freeze-thaw cycles. Three fluorescence components of DOM in the forest soil leachates were identified as humic acid-like DOM, fulvic acid-like DOM and protein-like DOM. The DOM components of BKPF soil leachates were mainly consisted of fulvic acid-like substances with a high humification index. However, the DOM from WBF soil leachates was dominated by humic acid-like substances with low stability, and the three fluorescence components were significantly affected by the freeze-thaw intensity. Results from the redundancy analysis showed that under the experimental conditions, forest type played a leading role in changing DOM properties. The DOM content and its three fluorescence intensities of WBF soil leachates were higher than those of BKPF. Soil moisture significantly affected the aromaticity of DOM in the forest soil leachates, and the DOM aromaticity of soil leachates from the two forest stands ranked as medium moisture > high moisture > low moisture. With the increases of freeze-thaw intensity, the DOM aromaticity of BKPF soil leachates significantly decreased. Furthermore, the increases of freeze-thaw cycles significantly increased the humification degree of DOM in the forest soil leachates. Therefore, upon different freeze-thaw disturbance, the DOM content and bioavailability of soil leachates with low moisture tended to increase, particularly in the WBF soil leachates, which may result in an increased lea-ching of DOM in temperate forest soils during spring freeze-thaw periods. The results provide a refe-rence for further investigating DOM turnover in temperate forest soils during spring freeze-thaw periods.