Growth of vegetative cells and germination of endospores of Sporolactobacillus at reduced water activity (aw) values were studied with NaCl and glycerol as humectants, and compared with Bacillus cereus and Clostridium sporogenes. With NaCl the highest aw for complete inhibition of both growth of vegetative cells and germinated spores of Sporolactobacillus was 0.955. With glycerol, growth of vegetative cells was completely inhibited at 0.905 as compared to 0.880 for vegetative growth resulting from germinated endospores. The mechanism of this phenomenon cannot be explained yet. NaCl appeared to have a toxic effect, in addition to inhibition by reduced aw per se.
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