Alkaloids are widely present in food and medicine. However, many alkaloids have poisoning effect. Thus, a sensitive and rapid method for determination of alkaloids is highly desired. In this study, a convenient, reliable and accurate method is developed for the simultaneous determination of 16 poisonous alkaloids in human blood by ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with supported liquid extraction (SLE). The 16 alkaloids include colchicine, anisodamine, scopolamine, atropine, sinomenine, dicaine, thebaine, papaverine, strychnine, brucine, aconitine, hypaconitine, yunaconitine, deoxyaconitine, bullatine A and bullatine B. Low limits of detection (LODs) were obtained ranging in 0.002-0.03 μg L. The linear ranges are 0.005-200 μg L for colchicine, papaverine, brucine, aconitine, hypaconitine, yunaconitine and deoxyaconitine, 0.01-200 μg L for anisodamine and scopolamine, 0.05-200 μg L for atropine, strychnine, bullatine A and bullatine B, 0. 1-200 μg L for other target analytes, all with correlation coefficients (R) higher than 0.995. The recoveries range from 79.2% to 95.8%. The relative standard deviation (RSD) values are lower than 15% and the values of matrix effect (ME) range in 86.3%-118.2%. This method of UPLC-MS/MS coupled with SLE has high potential for analyzing multiple alkaloids in blood samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jchromb.2019.121789 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Acceptability and preliminary outcomes of the Enhanced Assess, Acknowledge, and Act Sexual Assault Resistance Education Program for women who use alcohol and cannabis (EAAA+): Findings from an open pilot trial.
J Stud Alcohol Drugs
January 2025
Department of Psychology/Women's & Gender Studies within Interdisciplinary and Critical Studies, University of Windsor.
Objective: One in five college women experiences sexual assault (SA). Feminist scholars have called for the use of programming that empowers women by increasing their ability to recognize and resist SA. One such program, the Enhanced Assess, Acknowledge, Act SA Resistance Education Program (EAAA), has demonstrated lower rates of SA up to 24 months (Senn et al.
View Article and Find Full Text PDFTranscriptome Analysis Reveals the Key Genes and Pathways for Growth, Ion Transport, and Oxidative Stress in Post-Larval Black Tiger Shrimp (Penaeus monodon) Under Acute Low Salt Stress.
Mar Biotechnol (NY)
January 2025
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China.
As an abiotic stress factor, salinity significantly affects the physiological activities of crustaceans. In this study, transcriptome sequencing was used to evaluate the mechanism of ion transport and the physiological response of black tiger shrimp (Penaeus monodon) under low salt stress. Four hundred post larval (PL) stage P.
View Article and Find Full Text PDFNMR Spectral Editing, Water Suppression, and Dipolar Decoupling in Low-Field NMR Spectroscopy Using Optimal Control Pulses and Multiple-Pulse Sequence.
Anal Chem
January 2025
Experimental Physics III, TU Dortmund University, Dortmund 44227, Germany.
Spectral dispersion in low-field nuclear magnetic resonance (NMR) can significantly affect NMR spectral analysis, particularly when studying complex mixtures like metabolic profiling of biological samples. To address signal superposition in these spectra, we employed spectral editing with selective excitation pulses, proving it to be a suitable approach. Optimal control pulses were implemented in low-field NMR and demonstrated their capability to selectively excite and eliminate specific amino acids, such as phenylalanine and taurine, either individually or simultaneously.
View Article and Find Full Text PDFSimultaneous Activation of Beta-Oxidation and De Novo Lipogenesis in MASLD-HCC: A New Paradigm.
Liver Int
February 2025
Department of Digestive and Hepatobiliary Medicine, CHU Clermont-Ferrand, Clermont-Ferrand, France.
Background And Aims: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common cause of hepatocellular carcinoma (HCC). In this study, we combine metabolomic and gene expression analysis to compare HCC tissues with non-tumoural tissues (NTT).
Methods: A non-targeted metabolomic strategy LC-MS was applied to 52 pairs of human MASLD-HCC and NTT separated into 2 groups according to fibrosis severity F0F1-F2 versus F3F4.
Background: The grieving process caused by the loss of a loved one triggers a range of responses. While most people experience adaptive grief, some may experience intense distress and persistent symptoms. Prolonged Grief Disorder is commonly diagnosed using the ICD-11 and the DSM-5-TR.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!