Identification and Phylogenetic Classification of species Isolated from Sheep and Cattle by PCR-RFLP in Zabol, in Sistan and Baluchistan Province, Southeast Iran.

Background: The detection of species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related genotypes by restriction fragment length polymorphism PCR.

Methods: Seventy adult flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, south-east Iran (Jan-Jul 2017). species were determined based on molecular features. For molecular detection, ITS1 region was amplified and sequenced. A 700 bp fragment was amplified. These were digested with RasΙ enzyme. specific fragments were 47, 59, 68, 104, and 370, while those related to had 45, 55, 170, 370.

Results: The two main species of and are responsible for fasciolosis in sheep and cattle in our region. From 35 isolated from cattle, 3 and 32 were and respectively. From 35 isolated from sheep, 4 were and 31 were

Conclusion: All Seventy samples from two different hosts (cattle and sheep) were identified as either or by PCR-RFLP. Genotypic variability of species was high in our region. It is recommended to assess molecular variation of isolates in other host livestock.