AI Article Synopsis
- Calcium ions are crucial for maintaining energy balance in heart muscle, with differences observed in mitochondrial calcium handling between heart failure with preserved ejection fraction (HFpEF) and reduced ejection fraction (HFrEF).
- In a study on ZSF1-rat models, ZSF1-obese rats showed higher resting mitochondrial calcium levels and larger responses to stimulation compared to ZSF1-lean controls, indicating altered calcium dynamics in HFpEF.
- These changes in mitochondrial and cytosolic calcium handling may help maintain ATP supply during mild dysfunction, but chronic high mitochondrial calcium levels could lead to harmful cellular processes.
Article Abstract
Aim: Calcium ions play a pivotal role in matching energy supply and demand in cardiac muscle. Mitochondrial calcium concentration is lower in animal models of heart failure with reduced ejection fraction (HFrEF), but limited information is available about mitochondrial calcium handling in heart failure with preserved ejection fraction (HFpEF).
Methods: We assessed mitochondrial Ca handling in intact cardiomyocytes from Zucker/fatty Spontaneously hypertensive F1 hybrid (ZSF1)-lean (control) and ZSF1-obese rats, a metabolic risk-related model of HFpEF. A mitochondrially targeted Ca indicator (MitoCam) was expressed in cultured adult rat cardiomyocytes. Cytosolic and mitochondrial Ca transients were measured at different stimulation frequencies. Mitochondrial respiration and swelling, and expression of key proteins were determined ex vivo.
Results: At rest, mitochondrial Ca concentration in ZSF1-obese was larger than in ZSF1-lean. The diastolic and systolic mitochondrial Ca concentrations increased with stimulation frequency, but the steady-state levels were larger in ZSF1-obese. The half-widths of the contractile responses, the resting cytosolic Ca concentration and the decay half-times of the cytosolic Ca transients were higher in ZSF1-obese, likely because of a lower SERCA2a/phospholamban ratio. Mitochondrial respiration was lower, particularly with nicotinamide adenine dinucleotide (NADH) (complex I) substrates, and mitochondrial swelling was larger in ZSF1-obese.
Conclusion: The free mitochondrial calcium concentration is higher in HFpEF owing to alterations in mitochondrial and cytosolic Ca handling. This coupling between cytosolic and mitochondrial Ca levels may compensate for myocardial ATP supply in vivo under conditions of mild mitochondrial dysfunction. However, if mitochondrial Ca concentration is sustainedly increased, it might trigger mitochondrial permeability transition pore opening.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064935 | PMC |
| http://dx.doi.org/10.1111/apha.13378 | DOI Listing |
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