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MUC20 expression marks the receptive phase of the human endometrium. | LitMetric

MUC20 expression marks the receptive phase of the human endometrium.

Reprod Biomed Online

Competence Centre on Health Technologies, Tiigi 61b, Tartu 50410, Estonia; Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, Tartu 50406, Estonia; Department of Biomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, Tartu 50411, Estonia; Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Haartmaninkatu 2, Helsinki 00014, Finland. Electronic address:

Published: November 2019

AI Article Synopsis

  • Researchers wanted to know how a protein called MUC20 changes in different types of cells in the uterus during the menstrual cycle.
  • They looked at data from various studies and used tests to see how much MUC20 is present in the cells during two different phases of the cycle.
  • They discovered that MUC20 is mostly found in certain cells when the uterus is ready to receive a fertilized egg, suggesting it could be an important marker for understanding fertility.

Article Abstract

Research Question: How does mucin MUC20 expression change during the menstrual cycle in different cell types of human endometrium?

Design: Study involved examination of MUC20 expression in two previously published RNA-seq datasets in whole endometrial tissue (n = 10), sorted endometrial epithelial (n = 44) or stromal (n = 42) cell samples. RNA-Seq results were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in whole tissue (n = 10), sorted epithelial (n = 17) and stromal (n = 17) cell samples. MUC20 protein localization and expression were analysed in human endometrium by immunohistochemical analysis of intact endometrial tissue (n = 6) and also Western blot of cultured stromal and epithelial cells (n = 2).

Results: MUC20 is differentially expressed in the endometrium between the pre-receptive and receptive phases. We show that MUC20 is predominantly expressed by epithelial cells of the receptive endometrium, both at the mRNA (RNA-Seq, P = 0.005; qRT-PCR, P = 0.039) and protein levels (Western blot; immunohistochemistry, P = 0.029).

Conclusion: Our results indicate MUC20 as a novel marker of mid-secretory endometrial biology. We propose a model of MUC20 function in the hepatocyte growth factor (HGF)-activated mesenchymal-epithelial transition (MET) receptor signalling specifically in the receptive phase. Further investigations should reveal the precise function of MUC20 in human endometrium and the possible connection between MUC20 and HGF-activated MET receptor signalling. MUC20 could potentially be included in the list of endometrial receptivity markers after further clinical validation.

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Source
http://dx.doi.org/10.1016/j.rbmo.2019.05.004DOI Listing

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