Synthesis and exploration of a novel chlorobenzylated 2-aminothiazole-phenyltriazole hybrid as migratory inhibitor of B16F10 in melanoma cells.

In the study presented here, a novel chlorobenzylated bi-heterocyclic hybrid molecule () was synthesized and its structural confirmation was carried out by IR, H-NMR, C-NMR and CHN analysis data. This compound was subjected to biological study with B16F10 mouse melanoma cells. The anti-proliferative results showed that showed no significant toxicity at concentrations ranging of 0-44 μM. The treatment of B16F10 cells with at aforementioned concentration range indicated that migration of cells was significantly lower than that of the control cells in a dose dependent manner. The possible migration inhibitory effect of these melanoma cells was further evaluated through gelatinolytic activity of MMP-2 and MMP-9 secreted from B16F10 cells. It was inferred from our results that was not affecting the expression and activity of these enzymes. Some other zinc-dependent matrix metalloproteinases (MMPs) were involved in the inhibitory progression. Taken together, compound 7 inhibited migrations of B16F10 mouse melanoma cells. Therefore, it may deserve consideration as a potential agent for the treatment of cancer.