AI Article Synopsis
- l-lactate is crucial for metabolism and signaling in both eukaryotes and bacteria.
- Monocarboxylate transporters (MCTs) from the SLC16 family facilitate l-lactate transport across cell membranes.
- A new protocol has been developed for expressing and purifying a related protein in large quantities, which is stable and suitable for further biochemical and structural research.
Article Abstract
l-lactate plays an important role as metabolite and signaling molecule in eukaryotes and bacteria. Monocarboxylate transporters (MCTs) of the SLC16 solute carrier family are responsible for the transport of l-lactate across eukaryotic and bacterial cell membranes. Here we report an efficient protocol for the expression and purification of an SLC16 family homologue in milligram amounts. The purified protein is stable and can thus be used for biochemical and structural studies as shown by successful crystallization.
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| http://dx.doi.org/10.1016/j.pep.2019.105484 | DOI Listing |
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