[Diagnostic and prognostic significance of the hypoxia-dependent factor-1α for the development of a carious lesion].

The research objective was to study the diagnostic value of a hypoxia-dependent factor (HIF-1α) in gingival fluid for assessment of carious process risk. 75 patients with dentine caries entered were enrolled in the study: group 1 (n=30) - primary caries, group 2 (n=45) - secondary recurrent disease (12 months after treatment). HIF-1α was assessed in in gingival liquid of all patients before treatment by the IFA method. Statistical analysis included logistic regression and the ROC analysis. In the 2 group of patients HIF-1α value was two-fold higher than in group 1 (p<0.001) and by 82% in healthy controls (p<0.001). The ROC analysis revealed that the initial HIF-1α level higher than 98 pg/ml was associated with high risk of caries relapse in 12 months after treatment (with diagnostic sensitivity of 88.4% and specificity of 78.1%), and chances of disease development are increased by 4 times (p<0.0001). Logistic regressions created a mathematical model for calculation of caries relapse prognosis. Pearson's χ=50.3 (p<0.0001) indicated the statistical importance of dependence between the risk and the size of a predictor. HIF-1α concentration change in gingival liquid from 80 pg/ml to 170 pg/ml correlates with the 12 months recurrent disease risk growth from 13 up to 94%. Thus, the initial gingival fluid content of HIF-1α higher than 98 pg/ml indicates high risk for caries relapse and demands personification of preventive measures of the disease progression.