Induced pluripotent stem (iPS) cells are important tools for studying differentiation and for use in patient-specific disease modeling. We present a detailed method for the reprogramming of primary human fibroblasts to induced pluripotent stem cells using Sendai virus. These procedures allow for the efficient generation of multiple high-quality feeder-independent iPS cell lines for a given human fibroblast line. The iPS cell lines generated by this protocol can be used in a variety of differentiation and gene expression studies, as well as in genetic manipulations.
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| http://dx.doi.org/10.1007/978-1-4939-9837-1_18 | DOI Listing |
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