AI Article Synopsis
- Genetic modification of mouse embryonic stem (ES) cells is crucial for creating various mutant mouse lines to explore gene function and mammalian biology.!
- The text outlines ES cell culture and transfection methods for editing the ES cell genome to create specific targeted clones.!
- It discusses traditional gene targeting techniques along with the CRISPR/Cas9 system, which enhances the efficiency of this process by creating double-strand DNA breaks at designated locations in the genome.!
Article Abstract
Genetic modification of mouse embryonic stem (ES) cells is a powerful technology that enabled the generation of a plethora of mutant mouse lines to study gene function and mammalian biology. Here we describe ES cell culture and transfection techniques used to manipulate the ES cell genome to obtain targeted ES cell clones. We include the standard gene targeting approach as well as the application of the CRISPR/Cas9 system that can improve the efficiency of homologous recombination in ES cells by introducing a double-strand DNA break at the target site.
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| http://dx.doi.org/10.1007/978-1-4939-9837-1_5 | DOI Listing |
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