Ultra-compact miniaturized optical components for microendoscopic tools and miniaturized microscopes are required for minimally invasive imaging. Current microendoscopic technologies used for deep tissue imaging procedures are limited to a large diameter and/or low resolution due to manufacturing restrictions. We demonstrate a platform for miniaturization of an optical imaging system for microendoscopic applications with a resolution of 1 µm. We designed our probe using cascaded micro-lenses and waveguides (lensguide) to achieve a probe as small as 100 µm x 100 µm with a field of view of 60 µm in diameter. We demonstrate wide-field microscopy based on our polymeric probe fabricated using photolithography and a two-photon polymerization process.
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http://dx.doi.org/10.1364/OE.27.022352 | DOI Listing |
Ann Plast Surg
February 2025
From the Department of Plastic and Reconstructive Surgery, Ewha Womans University College of Medicine, Mokdong Hospital, Seoul, Republic of Korea.
Indocyanine green (ICG) is a water-soluble green substance that is detectable through infrared cameras and emits greenish light. Approved for medical use in the 1950s, ICG has gained prominence as a real-time visualization tool. Widely recognized as a generally safe substance, ICG is applied in diverse fields.
View Article and Find Full Text PDFAnal Chem
January 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
The near-infrared electrochemiluminescence (NIR-ECL) technique has received special attention in cell imaging and biomedical analysis due to its deep tissue penetration, low background interference, and high sensitivity. Although cyanine-based dyes are promising NIR-ECL luminophores, limited ECL efficiency and the need for exogenous coreactants have prevented their widespread application. In this work, poly[9,9-bis(3'-(-dimethylamino)propyl)-2,7-fluorene]--2,7-(9,9-dioctylfluorene)] (PFN) was innovatively developed to significantly invigorate the NIR-ECL performance of heptamethine cyanine dye IR 783 by the resonance energy transfer (RET) strategy.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO 80305.
Immunological interventions, like vaccinations, are enabled by the predictive control of humoral responses to novel antigens. While the development trajectories for many broadly neutralizing antibodies (bnAbs) have been measured, it is less established how human subtype-specific antibodies develop from their precursors. In this work, we evaluated the retrospective development trajectories for eight anti-SARS-CoV-2 Spike human antibodies (Abs).
View Article and Find Full Text PDFFront Vet Sci
January 2025
College of Veterinary Medicine, Michigan State University, East Lansing, MI, United States.
Veterinary intervention in zoological species can be complicated by species-specific social dynamics. African wild dogs are a pack species and removal or separation of an individual may disrupt established pack hierarchy resulting in conspecific aggression. Therefore, medical interventions that optimize a quick return to health are ideal to minimize the duration of absence from the pack.
View Article and Find Full Text PDFJ Biomed Opt
January 2025
Columbia University, Department of Electrical Engineering, New York, United States.
Significance: Radiofrequency ablation to treat atrial fibrillation (AF) involves isolating the pulmonary vein from the left atria to prevent AF from occurring. However, creating ablation lesions within the pulmonary veins can cause adverse complications.
Aim: We propose automated classification algorithms to classify optical coherence tomography (OCT) volumes of human venoatrial junctions.
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