[The development of polymerase chain reaction diagnostic of colorectal cancer based on DNA integrity separated from feces of patient.].

The article presents procedure of diagnostic of colorectal cancer on the basis of analysis of integrity of fecal DNA. The samples of stool were picked out in 91 patients and out of them: 60 patients with colorectal cancer, 24 healthy volunteers (control group) and 7 patients with benign tumors. The evaluation of integrity of genome DNA using technique of polymerase chain reaction analysis specified involvement of two long fragments - sites of genes TP53 and MLH1. In stool of 47 patients with colorectal cancer the long fragments of DNA were detected while analysis of DNA from stool of individuals from control group detected only short fragments of DNA with size less than 200 np. The technique of non-invasive polymerase chain reaction diagnostic of colorectal cancer was developed. The sensitivity and specificity of this technique made up to 78% and 100% (p<0.0001). The sensitivity of technique has no significant differences for patients with different stages of disease that permits to apply it for early diagnostic of colorectal cancer.