In this paper, we have cultured normal epithelial cells (HaCaT) as analytes to detect the sensitivity of a biosensor based on Fano resonance metamaterials (FRMMs). The frequency shift of the transmission spectrum was experimentally measured at three different concentrations (0.2×10, 0.5×10, and 5×10 /) of HaCaT cells. By employing the FRMMs-based biosensor, the detection concentration of HaCaT cells can approximately arrive at 0.2×10 /; further, the corresponding is 25 GHz, which reaches the measurement limit of the THz-TDS system. Additionally, the increase of HaCaT cell concentration causes a different redshift of from 24-50 GHz, and the maximum of can reach 50 GHz when the HaCaT cell concentration is at 5×10 cell/ml. Similarly, the simulated results show that the depends on the numbers of analytes with a semiball shape and the refractive index of analytes. The theoretical sensitivity was calculated to be 481 GHz/RIU. The proposed FRMMs-based biosensor paves a fascinating platform for biological and biomedical applications and may become a valuable complementary reference for traditional biological research.
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