A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped and aerobic bacterial strain (designated lm1) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong province, PR China). Strain lm1 grew between 15 and 45 °C (optimum, 40 °C). The pH range for growth was at pH 4.0-10.0 (optimum growth at pH 8.0). The range of NaCl concentration for the growth of strain lm1 was 0-4.0 % (w/v), with optimum growth at 1.0-2.0 % (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain lm1 belonged to the genus and was closely related to JCM 16873 (96.7 % 16S rRNA gene sequence similarity) and CCTCC AB2013083 (96.2 %). The average nucleotide identity value and the digital DNA-DNA hybridization value between strain lm1 and JCM 16873 were 73.4 and 14.3 %, respectively. The major cellular fatty acids of strain lm1 were Ccyclo ω8, Cω7 and C. Strain lm1 contained Q-10 as the predominant respiratory quinone. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and five unknown lipids. The G+C content of the genomic DNA was 64.4 mol%. Based on polyphasic taxonomic data, strain lm1 could be classified as a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is lm1 (=CCTCC AB2019071=KCTC 72156).
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http://dx.doi.org/10.1099/ijsem.0.003691 | DOI Listing |
Antonie Van Leeuwenhoek
December 2024
Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, South Korea.
A Gram-stain-negative, aerobic, non-spore-forming, non-motile, coccus-shaped, and red-pigmented bacterial strain designated as CJ14 was isolated from lettuce cultivation soil in Yong-In, South Korea. Strain CJ14 grew optimally on Luria-Bertani agar at 37 ℃ and pH 7.0 in the absence of NaCl.
View Article and Find Full Text PDFBiochem Biophys Rep
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Durgapur Degree College, Rajshahi 6240, Bangladesh.
This research aims to isolate, characterize, and analyze pure compounds from leaf extract to investigate its antibacterial, antidiabetic, cytotoxic, and phytotoxic effects. Fresh leaves were collected, dried, and subjected to methanol extraction to obtain a crude extract. From the petroleum ether fraction (PEF) of this extract, three fractions-designated LM1, LM2, and LM3-were prepared using column chromatography.
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June 2024
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
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View Article and Find Full Text PDFMicroorganisms
February 2024
School of Ocean, Yantai University, Yantai 264005, China.
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January 2024
Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Ermita, Manila 1000, Philippines.
Micro-organisms belonging to the genus complex are often used for oral consumption and are generally considered safe but can exhibit pathogenicity in rare and specific cases. Therefore, screening and understanding genetic factors that may contribute to pathogenicity can yield valuable insights regarding probiotic safety. LM1, SK151, BS25, SK152 and PF01 are current probiotics of interest; however, their safety profiles have not been explored.
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