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Sequence-Specific Mucins for Glycocalyx Engineering. | LitMetric

Sequence-Specific Mucins for Glycocalyx Engineering.

ACS Synth Biol

Field of Biophysics , Cornell University, Ithaca , New York 14853 , United States.

Published: October 2019

AI Article Synopsis

Article Abstract

Few approaches exist for the stable and controllable synthesis of customized mucin glycoproteins for glycocalyx editing in eukaryotic cells. Taking advantage of custom gene synthesis and a biology-by-parts approach to cDNA construction, we build a library of swappable DNA bricks for mucin leader tags, membrane anchors, cytoplasmic motifs, and optical reporters, as well as codon-optimized native mucin repeats and newly designed domains for synthetic mucins. We construct a library of over 50 mucins, each with unique chemical, structural, and optical properties and describe how additional permutations could readily be constructed. We apply the library to explore sequence-specific effects on glycosylation for engineering of mucins. We find that the extension of the immature α-GalNAc Tn-antigen to Core 1 and Core 2 glycan structures depends on the underlying peptide backbone sequence. Glycosylation could also be influenced through recycling motifs on the mucin cytoplasmic tail. We expect that the mucin parts inventory presented here can be broadly applied for glycocalyx research and mucin-based biotechnologies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7205180PMC
http://dx.doi.org/10.1021/acssynbio.9b00127DOI Listing

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