Bisphosphonate activation of crystallized bioglass scaffolds for enhanced bone formation.

Mater Sci Eng C Mater Biol Appl

Department of Materials Science & Engineering, Monash University, Clayton, Victoria 3800, Australia. Electronic address:

Published: November 2019

The interplay between bone formation by osteoblasts and bone resorption by osteoclasts has a critical effect on bone remodelling processes, and resultant bone quality. Bone scaffolds combined with anti-resorptive bisphosphonate drugs are a promising approach to achieving bone regeneration. Here, we have examined the synergistic effects of the bisphosphonate alendronate (ALD) coated onto calcium phosphate (CaP) modified, sintered bioactive glass 45S5 (BG) scaffolds, on osteoblast stimulation and osteoclast inhibition. After BG pre-treatment with ALD (10 M) for 5 days, human MG-63 osteoblasts displayed increased cellular proliferation and significantly enhanced alkaline phosphatase activity (ALP), in comparison with a non-ALD control BG. In contrast, human THP-1-derived osteoclasts cultured with 10 M ALD pretreated BG scaffolds showed a significant decrease in tartrate-resistant acid phosphatase (TRAcP) activity, and morphological changes indicative of functional inhibition, including reduced cell size and disruption of the osteoclast sealing zone (F-actin rings). These findings indicate that ALD-coated BG scaffolds promote osteoblast activity and inhibit osteoclast function to enhance bone formation.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.msec.2019.109937DOI Listing

Publication Analysis

Top Keywords

bone formation
12
bone
8
scaffolds
5
bisphosphonate activation
4
activation crystallized
4
crystallized bioglass
4
bioglass scaffolds
4
scaffolds enhanced
4
enhanced bone
4
formation interplay
4

Similar Publications

Bone Marrow-derived NGFR-positive Dendritic Cells Regulate Arterial Remodeling.

Am J Physiol Cell Physiol

January 2025

Department of Cardiovascular Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan.

It has been proposed that bone marrow contributes to the pathogenesis of arteriosclerosis. Nerve growth factor receptor (NGFR) is expressed in bone marrow stromal cells; it is also present in peripheral blood and ischemic coronary arteries. We hypothesized that bone marrow-derived NGFR-positive (NGFR) cells regulate arterial remodeling.

View Article and Find Full Text PDF

Agenesis of maxillary lateral incisors: Bone formation by orthodontic tooth movement and long-term stability of the edentulous alveolar ridge at 12-15 years after treatment.

Am J Orthod Dentofacial Orthop

January 2025

Department of Orthodontics, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic; Department of Dentistry, First Faculty of Medicine, Charles University and the General University Hospital, Prague, Czech Republic. Electronic address:

Introduction: The primary aim of this study was to assess the amount and long-term stability of orthodontically created bone in patients with agenesis of maxillary lateral incisors after canine distalization. The secondary aim was to explore the impact of patient age on the process of alveolar bone resorption.

Methods: A group of patients with agenesis of the maxillary permanent lateral incisor was examined at 4 time points: the beginning of orthodontic treatment (T1, n = 80), the end of treatment (T2, n = 80), 2-5 years after treatment (T3, n = 79), and 12-15 years after treatment (T4, n = 32).

View Article and Find Full Text PDF

Introduction: Diffuse pulmonary ossification (DPO) refers to the unusual formation of mature bone tissue within the lung parenchyma. It has been shown to be associated with a number of cardiac and chronic lung diseases. The relation between DPO and idiopathic pulmonary fibrosis (IPF) has been shown in the literature.

View Article and Find Full Text PDF

Clonal hematopoiesis JAKs up plaque formation.

J Clin Invest

January 2025

Department of Medicine, Division of Hematology/Oncology, and.

Clonal hematopoiesis (CH) is a condition in which hematopoietic stem cells (HSCs) acquire mutations seen in leukemia. While individuals with CH generally do not show signs of hematologic disease, the condition becomes more common with age and correlates with age-related diseases, especially cardiovascular disease (CVD). JAK2 mutations in HSCs can lead to CH and correlate with atherosclerosis, but the condition has been difficult to study because of challenges modeling the mutant cells at very low frequency.

View Article and Find Full Text PDF

Advancements in GelMA bioactive hydrogels: Strategies for infection control and bone tissue regeneration.

Theranostics

January 2025

Department of Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, China.

Infectious bone defects present a significant clinical challenge, characterized by infection, inflammation, and subsequent bone tissue destruction. Traditional treatments, including antibiotic therapy, surgical debridement, and bone grafting, often fail to address these defects effectively. However, recent advancements in biomaterials research have introduced innovative solutions for managing infectious bone defects.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!