Cytotoxic Effects of Ferula Latisecta on Human Glioma U87 Cells.

Glioblastoma multiforme (GBM) is the fatal type of astrocytic tumors with a survival rate of 12 months. The present study, for the first time, evaluated the cytotoxic impacts of () hydroalcoholic extract on U87 GBM cell line. The MTT assay measured the cellular toxicity following 24- and 48 h treatment with various doses of (0-800 μg/mL). Apoptosis was evaluated by an Annexin V/propidium iodide (PI) staining 24 h after treatment by . Moreover, to determine the cellular metastasis of U87 cells, we used a gelatin zymography assay (matrix metalloproteinase [MMP]-2/-9 enzymatic activity). The outcomes showed that mitigated the viability of U87 cells in a concentration- and time-dependent manner with IC values of 145.3 and 192.3 μg/mL obtained for 24- and 48 h treatments, respectively. induced apoptosis in a concentration-dependent manner after 24 h. Also, MMP-9 activity was significantly decreased following 24 h after treatment concentration-dependently with no change in MMP-2 enzymatic activity. This study showed that induced cytotoxicity and apoptosis, and mitigated metastasis of U87 GBM cells. Hence, could be beneficial as a promising natural herb against GBM after further studies.