Using intracellular microelectrode recordings, it was observed that FSH caused the hyperpolarization of cultured Sertoli cells from the testis of 17-day old rats; this hyperpolarization was related to the activation of a Na/K pump and an increase in Na/Ca influx and K efflux. The effect was irreversible and mediated by cAMP. Some other results suggested an initial effect of FSH on the junctional complex between the Sertoli cells and then its calcium dependence. Voltage-clamped membrane currents were investigated from whole-cell patch clamp recordings performed on single Leydig cells isolated from adult rat testis. Two outward currents were evoked by depolarizing voltage steps: a potassium current recorded in cells dialyzed with low-calcium media (10(-9)-10(-8) M) and a chloride current recorded in cells dialyzed with high-calcium media (10(-7)-10(-6) M). These responses to modifications of intracellular calcium were not affected by hCG. However, this hormone induced an early change of the K current to a Cl current when the cells were dialyzed by a low-buffered EGTA-calcium medium. These results suggest that hCG stimulation involved the blockade of a potassium current and the activation of a chloride current through an increase of intracellular calcium.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1051/rnd:19880703 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hormonal Regulation of Urokinase- and Tissue-Type Plasminogen Activator in Mouse Sertoli Cells.
Mol Reprod Dev
January 2025
Department of Anatomy, Histology, Forensic Medicine and Orthopedic, Section of Histology, Sapienza University of Rome, Rome, Italy.
A role for the plasminogen activator (PA) system has been postulated in mammalian gonads, considering the complex process of morphogenesis these organs undergo during their development. Our results show that mouse Sertoli cells under basal conditions produce both types of PA, tissue-type PA (tPA) and urokinase-type PA (uPA), and hormonal treatments increase the production of both enzymes. The increased enzyme secretion does not correlate with a parallel increase in their mRNAs.
View Article and Find Full Text PDFThe action of retinoic acid on spermatogonia in the testis.
Curr Top Dev Biol
January 2025
School of Molecular Biosciences, Washington State University, Pullman, Washington, United States. Electronic address:
For mammalian spermatogenesis to proceed normally, it is essential that the population of testicular progenitor cells, A undifferentiated spermatogonia (A), undergoes differentiation during the A to A1 transition that occurs at the onset of spermatogenesis. The commitment of the A population to differentiation and leaving a quiescent, stem-like state gives rise to all the spermatozoa produced across the lifespan of an individual, and ultimately determines male fertility. The action of all-trans retinoic acid (atRA) on the A population is the determining factor that induces this change.
View Article and Find Full Text PDFIdentification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis.
BMC Med Genomics
January 2025
Department of Surgery, Faculty of General of Medicine, Koya University, Koya, Kurdistan Region - F.R., KOY45, Iraq.
Background: During mammalian spermatogenesis, the cytoskeleton system plays a significant role in morphological changes. Male infertility such as non-obstructive azoospermia (NOA) might be explained by studies of the cytoskeletal system during spermatogenesis.
Methods: The cytoskeleton, scaffold, and actin-binding genes were analyzed by microarray and bioinformatics (771 spermatogenic cellsgenes and 774 Sertoli cell genes).
Fertility in transgender and gender diverse people: systematic review of the effects of gender-affirming hormones on reproductive organs and fertility.
Hum Reprod Update
January 2025
Amsterdam UMC, Location Vrije Universiteit Amsterdam, Centre of Expertise on Gender Dysphoria, Amsterdam, The Netherlands.
Background: Transgender and gender diverse (TGD) people seek gender-affirming care at any age to manage gender identities or expressions that differ from their birth gender. Gender-affirming hormone treatment (GAHT) and gender-affirming surgery may alter reproductive function and/or anatomy, limiting future reproductive options to varying degrees, if individuals desire to either give birth or become a biological parent.
Objective And Rationale: TGD people increasingly pursue help for their reproductive questions, including fertility, fertility preservation, active desire for children, and future options.
Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig.
Curr Issues Mol Biol
December 2024
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Spermatogenesis is an advanced biological process, relying on intricate interactions between somatic and germ cells in testes. Investigating various cell types is challenging because of cellular heterogeneity. Single-cell RNA sequencing (scRNA-seq) offers a method to analyze cellular heterogeneity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!