Background: Since patients with breakthrough varicella (BV) have mild symptoms, clinical diagnosis is difficult. In high vaccine coverage area, as BV occurs sporadically, point of care test is required for controlling varicella outbreak. In this study, the reliability of varicella zoster virus (VZV)-loop mediated isothermal amplification (LAMP) was evaluated for the rapid diagnosis of BV.
Study Design: A total of 328 swab samples collected from patients with suspected varicella were analyzed. For the laboratory diagnosis of varicella, VZV real-time PCR was carried out using DNA extracted from swab samples. Swab samples without DNA extraction were used for VZV-LAMP(direct-LAMP).
Results: VZV infection was diagnosed by real-time PCR in 285 cases, including 105 natural varicella cases and 180 BV cases. VZV DNA was detected in 250 (87.8%) of the 285 cases by direct-LAMP. The presence and duration of fever, number of skin eruptions, and VZV DNA load were significantly lower in BV than natural varicella. The sensitivity of direct-LAMP for the diagnosis of varicella and BV was 93.3% and 84.4%, respectively.
Conclusions: Direct LAMP was considered to be useful for rapid diagnosis of BV as it has several advantages such as low cost, ease and rapidity, as compared to real time PCR.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jcv.2019.07.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
UltraNet: Unleashing the Power of Simplicity for Accurate Medical Image Segmentation.
Interdiscip Sci
December 2024
College of Mathematics and Systems Science, Shandong University of Science and Technology, Qingdao, 266590, China.
The imperative development of point-of-care diagnosis for accurate and rapid medical image segmentation, has become increasingly urgent in recent years. Although some pioneering work has applied complex modules to improve segmentation performance, resulting models are often heavy, which is not practical for the modern clinical setting of point-of-care diagnosis. To address these challenges, we propose UltraNet, a state-of-the-art lightweight model that achieves competitive performance in segmenting multiple parts of medical images with the lowest parameters and computational complexity.
View Article and Find Full Text PDF[Leading symptom: motor manifestations with impaired consciousnes : Management of epileptic seizures and status epilepticus].
Med Klin Intensivmed Notfmed
December 2024
Neurologische Klinik, Standort Gießen, Universitätsklinikum Gießen und Marburg, Klinikstraße 33, 35392, Gießen, Deutschland.
Epileptic seizures, which are often accompanied by a reduction in vigilance, are a common emergency. Every first-time epileptic seizure should be investigated further. Particular attention should be paid to whether it is an acute symptomatic seizure, which is an acute event characterized by a metabolic disorder or acute cerebral damage within a certain period of time, or possibly epilepsy.
View Article and Find Full Text PDFNovel isothermal nucleic acid amplification method for detecting malaria parasites.
Appl Microbiol Biotechnol
December 2024
Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Malaria, a parasitic disease caused by Plasmodium spp. and transmitted by Anopheles mosquitoes, remains a major global health issue, with an estimated 249 million cases and 608,000 deaths in 2022. Rapid and accurate diagnosis and treatment are crucial for malaria control and elimination.
View Article and Find Full Text PDFFrequency of Bovine Respiratory Disease Complex Bacterial and Viral Agents Using Multiplex Real-Time qPCR in Quebec, Canada, from 2019 to 2023.
Vet Sci
December 2024
Biovet Inc., Division of Antech Diagnostics and Mars Petcare Science & Diagnostics Company, Saint-Hyacinthe, QC J2S 8W2, Canada.
The bovine respiratory disease complex (BRD) is a multifactorial disease caused by various bacterial and viral pathogens. Using rapid pathogen detection techniques is helpful for tailoring therapeutic and preventive strategies in affected animals and herds. The objective of this study was to report the frequency of 10 pathogens by multiplex RT-qPCR on samples submitted for BRD diagnosis to a diagnostic laboratory (Biovet Inc.
View Article and Find Full Text PDFDiagnosis and Management of Kaposi Sarcoma-Associated Herpesvirus Inflammatory Cytokine Syndrome in Resource-Constrained Settings: A Case Report and an Adapted Case Definition.
Trop Med Infect Dis
December 2024
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Kaposi sarcoma-associated herpes virus (KSHV), also known as human herpes virus 8 (HHV-8), is the primary etiologic cause of Kaposi sarcoma (KS) and KSHV Inflammatory Cytokine Syndrome (KICS). Patients with KICS demonstrate symptoms of systemic inflammation, high KSHV viral load, elevation of inflammatory markers, and increased mortality. Management requires rapid diagnosis, treatment of underlying HIV, direct treatment of KS, and addressing the hyperimmune response.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!