The cleavage of the murine J kappa-segments by purified Ca/Mg-dependent endonuclease was studied. The enzyme was shown to cleave specifically the J kappa-segments introducing the double strand breaks in 5'-regions of the J kappa-genes. The number of thus generated discreet fragments does not change during incubation. Specificity of the enzyme is not changed by substitution of Ca/Mg for Mn. The enzyme activity is inhibited by 0.1 M NaCl as well as its specificity. Comparison of the enzyme with the known nuclease activities that cleave specifically the J kappa-segments demonstrated that the activities of the latter are determined by Ca/Mg-dependent endonuclease. The results suggest the possible participation of Ca/Mg-dependent endonuclease in Ig-genes recombination.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Induction of apoptosis by calcium D-glucarate in 7,12-dimethyl benz [a] anthracene-exposed mouse skin.
J Environ Pathol Toxicol Oncol
September 2007
Environmental Carcinogenesis Division, Industrial Toxicology Research Center, Mahatma Gandhi Marg, Lucknow, India.
Calcium glucarate (Cag), a naturally occurring nontoxic compound, suppresses the DMBA-induced tumor development in mouse skin. In the process of understanding the mechanisms of tumor suppression by Cag, we investigated the effect of topical application of Cag on selective and critical events of apoptotic pathway in DMBA-exposed mouse epidermis. Varied doses of DMBA or Cag were used for the study.
View Article and Find Full Text PDFGelatinases, endonuclease and Vascular Endothelial Growth Factor during development and regression of swine luteal tissue.
BMC Dev Biol
November 2006
Department of Veterinary Morphophysiology and Animal Production (DIMORFIPA), University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, Bologna, Italy.
Background: The development and regression of corpus luteum (CL) is characterized by an intense angiogenesis and angioregression accompanied by luteal tissue and extracellular matrix (ECM) remodelling. Vascular Endothelial Growth Factor (VEGF) is the main regulator of angiogenesis, promoting endothelial cell mitosis and differentiation. After the formation of neovascular tubes, the remodelling of ECM is essential for the correct development of CL, particularly by the action of specific class of proteolytic enzymes known as matrix metalloproteinases (MMPs).
View Article and Find Full Text PDF[Comparative study of Ca/Mg-dependent nucleases from the mammalian thymus].
Izv Akad Nauk Ser Biol
June 1998
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Ca/Mg-dependent nuclease is a possible key enzyme of apoptosis. We isolated and purified nucleases from the human and rat thymus to a homogeneous state and compared some properties of the obtained preparations with those of the earlier isolated Ca/Mg-dependent nuclease from the calf thymus. The activity of the nuclease from the human thymus in the presence of bivalent ions decreases in a sequence:(Ca + Mg) = (Ca + Mn) > Mn, that from the rat thymus: (Ca + Mn) > Mn > (Ca + Mg), and that from the calf thymus: (Ca + Mn) > (Ca + Mg) > Mn.
View Article and Find Full Text PDFDNA fragmentation and nuclear endonuclease activity in rat brain after severe closed head injury.
Brain Res
August 1996
University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Previous studies have suggested that brain cells undergo apoptotic cell death during several neurodegenerative disorders such as Alzheimer's disease, Parkinsonism and ischemic stroke. In the present study, apoptotic DNA fragmentation and activation of nuclear endonuclease were evaluated in rat brain cells after head trauma. Severe closed head injury was induced in rats by the impact of a 450-g weight dropped from a height of 2 m.
View Article and Find Full Text PDFTumor necrosis factor mediates apoptosis via Ca++/Mg++ dependent endonuclease with protein kinase C as a possible mechanism for cytokine resistance in human renal carcinoma cells.
J Urol
May 1996
Department of Urology, Mount Sinai School of Medicine, New York, 10029, USA.
Purpose: Because renal cell cancers have been found to be resistant to numerous chemotherapeutic agents, other agents including tumor necrosis factor are now being considered for clinical use. In this study, we used 2 renal cancer cell lines. SK-RC-42 and SK-RC-49, and determined the cytotoxic effects of tumor necrosis factor (TNF) and the possible mechanism of TNF resistance.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!