Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/09168451.2019.1659716 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Advancing osseointegration research: A dynamic three-dimensional (3D) culture model for dental implants.
J Dent Sci
January 2025
Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA, USA.
Background/purpose: studies are essential for understanding cellular responses, but traditional culture systems often neglect the three-dimensional (3D) structure of real implants, leading to limitations in cellular recruitment and behavior largely governed by gravity. The objective of this study was to pioneer a novel 3D dynamic osteoblastic culture system for assessing the biological capabilities of dental implants in a more clinically and physiologically relevant manner.
Materials And Methods: Rat bone marrow-derived osteoblasts were cultured in a 24-well dish with a vertically positioned dental implant.
Identifying a Biocontrol Bacterium with Disease-Prevention Potential and Employing It as a Powerful Biocontrol Agent Against .
Int J Mol Sci
January 2025
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Biocontrol microbes are environment friendly and safe for humans and animals. To seek biocontrol microbes effective in suppressing is important for tomato production. is a soil-borne pathogen capable of causing wilt in numerous plant species.
View Article and Find Full Text PDFBiocementation beyond the Petri dish, scaling up to 900 L batches and a meter-scale column.
Sci Rep
January 2025
Environmental Biotechnology, Institute of Microbiology, Department of Environment, Construction and Design, University of Applied Sciences and Arts of Southern Switzerland (SUPSI), 3 Medusoil SA, EPFL Innovation Park Building A, Manno, Switzerland.
Microbial-induced calcite precipitation (MICP), which leverages ureolytic microorganisms, has received significant attention during the past decade as a promising method for sustainable building and geoenvironmental applications. However, transitioning from lab-scale experimentation to volumes suitable for practical use poses challenges. This study addresses these obstacles by screening and analyzing over 50 strains sourced from (i) a natural environment in the canton of Ticino in Switzerland; (ii) microorganism banks; and (iii) an industry-scale bioreactor.
View Article and Find Full Text PDFWhy do people in the U.S. pay more for Bouillabaisse than Kaeng Som? Experimental tests of how cuisine and authenticity affect the value of food.
Appetite
January 2025
University of Minnesota Twin Cities, 75 East River Parkway Minneapolis, MN 55455, United States.
The average prices of foods vary across cuisines but do these differences reflect the value people assign to different cuisines or how much they are willing to pay? Across four preregistered randomized experiments (total N=1,083), we manipulated the cuisine-of-origin (French, Thai) and authenticity (low, high) descriptions for analogous dishes across cuisines (e.g., two seafood stews: French bouillabaisse and Thai kaeng som) and restaurant review pages in Studies 2-4.
View Article and Find Full Text PDFNondestructive Mechanical Characterization of Bioengineered Tissues by Digital Holography.
ACS Biomater Sci Eng
January 2025
Mechanical Engineering Department, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States.
Mechanical properties of engineered connective tissues are critical for their success, yet modern sensors that measure physical qualities of tissues for quality control are invasive and destructive. The goal of this work was to develop a noncontact, nondestructive method to measure mechanical attributes of engineered skin substitutes during production without disturbing the sterile culture packaging. We optimized a digital holographic vibrometry (DHV) system to measure the mechanical behavior of Apligraf living cellular skin substitute through the clear packaging in multiple conditions: resting on solid agar as when the tissue is shipped, on liquid media in which it is grown, and freely suspended in air as occurs when the media is removed for feeding.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!