AI Article Synopsis
- Intestinal pathogenic E. coli (InPEC) is a major cause of postweaning diarrhea (PWD) in pigs, with a study isolating 455 strains from affected pigs on large-scale farms between 2014 and 2016.
- Real-time PCR revealed that the most common virulence factor was the heat-labile enterotoxin (LT), followed by other toxins like heat-stable enterotoxin (STb), with significant variation based on location and year.
- A concerning finding was that 94.15% of the isolates were multidrug-resistant, highlighting the need for better strategies to combat InPEC-related diarrheal diseases in pigs.
Article Abstract
Intestinal pathogenic (InPEC) is a leading cause of postweaning diarrhea (PWD) in pigs. Here, a total of 455 strains were isolated from small intestinal content or feces from pigs with PWD in 56 large-scale (>500 sows; 10,000 animals per year) swine farms between 2014 and 2016. The frequency of occurrence of selected virulence factors for InPEC pathotypes was detected in 455 isolates by real-time PCR. Sequence types (STs), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility profiles of 171 isolates from 56 swine farms were further determined. The heat-labile enterotoxin (LT) was the most common (61.76%), followed by heat-stable enterotoxin (STb) (33.19%), (21.54%), STa (15.00%), (8.98%), (5.71%), (5.71%), F18 (3.25%), and F4 (2.25%) with rates varying by geographic area and year of isolation. Notably, hybrids of isolates were potentially more virulent, as some InPEC hybrids (virotype F18:LT::) can rapidly cause cell death in vitro. Genotypic analysis revealed that the most prominent genotype was ST10 (12.87%). The PFGE patterns were heterogeneous but were not ST or virotype related. A total of 94.15% of isolates were multidrug-resistant, with average resistance rates ranging from 90.05% for nalidixic acid to 2.34% for meropenem. Our investigation contributes to establishing the etiology of diarrhea and developing intervention strategies against -associated diarrheal disease in the future.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780709 | PMC |
| http://dx.doi.org/10.3390/microorganisms7090308 | DOI Listing |
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