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Biosynthesis of Two Hydroxybenzoic Acid-Amine Conjugates in Engineered . | LitMetric

Biosynthesis of Two Hydroxybenzoic Acid-Amine Conjugates in Engineered .

J Microbiol Biotechnol

Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 05029, Republic of Korea.

Published: October 2019

Two hydroxybenzoyl amines, 4-hydroxybenzoyl tyramine (4-HBT) and -2-hydroxybenzoyl tryptamine (2-HBT), were synthesized using . While 4-HBT was reported to demonstrate anti-atherosclerotic activity, 2-HBT showed anticonvulsant and antinociceptive activities. We introduced genes chorismate pyruvate-lyase (), tyrosine decarboxylase (), isochorismate synthase (), isochorismate pyruvate lyase (), and tryptophan decarboxylase () for each substrate, 4-hydroxybenzoic acid (4-HBA), tyramine, 2-hydroxybenzoic acid (2-HBA), and tryptamine, respectively, in . Genes for CoA ligase () and amide formation ( and ) were also introduced to form hydroxybenzoic acid and amine conjugates. In addition, we engineered to provide increased substrates. These approaches led to the yield of 259.3 mg/l 4-HBT and 227.2 mg/l 2-HBT and could be applied to synthesize diverse bioactive hydroxybenzoyl amine conjugates.

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http://dx.doi.org/10.4014/jmb.1907.07040DOI Listing

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