Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Two hydroxybenzoyl amines, 4-hydroxybenzoyl tyramine (4-HBT) and -2-hydroxybenzoyl tryptamine (2-HBT), were synthesized using . While 4-HBT was reported to demonstrate anti-atherosclerotic activity, 2-HBT showed anticonvulsant and antinociceptive activities. We introduced genes chorismate pyruvate-lyase (), tyrosine decarboxylase (), isochorismate synthase (), isochorismate pyruvate lyase (), and tryptophan decarboxylase () for each substrate, 4-hydroxybenzoic acid (4-HBA), tyramine, 2-hydroxybenzoic acid (2-HBA), and tryptamine, respectively, in . Genes for CoA ligase () and amide formation ( and ) were also introduced to form hydroxybenzoic acid and amine conjugates. In addition, we engineered to provide increased substrates. These approaches led to the yield of 259.3 mg/l 4-HBT and 227.2 mg/l 2-HBT and could be applied to synthesize diverse bioactive hydroxybenzoyl amine conjugates.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.4014/jmb.1907.07040 | DOI Listing |
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!