Protein identification from the parotoid macrogland secretion of .

J Venom Anim Toxins Incl Trop Dis

Laboratory of Biochemistry and Biophysics, Butantan Institute, São Paulo, SP, Brazil.

Published: August 2019

Background: Bufonid parotoid macrogland secretion contains several low molecular mass molecules, such as alkaloids and steroids. Nevertheless, its protein content is poorly understood. Herein, we applied a sample preparation methodology that allows the analysis of viscous matrices in order to examine its proteins.

Methods: parotoid macrogland secretion was submitted to ion-exchange batch sample preparation, yielding two fractions: salt-displaced fraction and acid-displaced fraction. Each sample was then fractionated by anionic-exchange chromatography, followed by proteomic analysis.

Results: Forty-two proteins could be identified, such as acyl-CoA-binding protein, alcohol dehydrogenase, calmodulin, galectin and histone. Moreover, analyses yielded 153 peptides, whereas BLAST analyses corroborated some of the proteomic-identified proteins. Furthermore, the peptide analyses indicate the presence of proteins related to apoptosis, cellular structure, catalysis and transport processes.

Conclusions: Proper sample preparation allowed the proteomic and identification of different proteins in the parotoid macrogland secretion. These results may increase the knowledge about the universe of molecules that compose amphibian skin secretion, as well as to understand their biological/physiological role in the granular gland.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6707386PMC
http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0029DOI Listing

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