Background: Reveal® Q+ for DON is an immunochromatographic test for quantitative determination of deoxynivalenol (DON) in grains.
Objective: A study was conducted to validate performance of this method for determination of DON in naturally contaminated corn and wheat and in DON-spiked corn/soy blend, soybeans, barley, malted barley, buckwheat, brown rice, sorghum, and distillers dried grain.
Methods: In addition to matrix testing, LOD, linearity, selectivity, robustness, and stability/lot-to-lot consistency testing were performed.
Results: The LOD was determined to be 0.014 ppm in corn and 0.037 ppm in wheat, and the LOQ 0.042 ppm in corn and 0.11 ppm in wheat. Recovery ranged from 90 to 104% across a range of reference values of 0.5 to 34.5 ppm. Linearity calculation comparing test results with reference values produced R2 values of 0.999 in both matrixes. Internal results with corn and wheat were corroborated in independent laboratory testing. For DON-spiked commodities, mean recovery across a range of DON concentration from 0.5 to 30 ppm ranged from 90 to 109%. Results of selectivity testing showed no cross-reactivity with other mycotoxins and no interference in detection of DON. Reagent lot-to-lot consistency and stability studies showed consistent results across a range of DON levels and established expiration dating for the test of 18 months after manufacture when stored under specified conditions. Conclusions and Highlights: The Reveal Q+ for DON test offers reliable performance as well as the advantages of aqueous sample extraction, procedural simplicity, minimal labor and equipment requirements, and rapid results.
Conclusions: The Reveal Q+ for DON test is validated as a Performace Tested Method in Corn, Wheat, and a variety of other grains.
Highlights: The test provides rapid results from a simple aqueous extraction and requires minimal labor and equipment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.5740/jaoacint.19-0252 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle-based CdTe quantum dots for sensitive zearalenone determination.
Mikrochim Acta
December 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China.
A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto FeO@SiO and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals.
View Article and Find Full Text PDFImpacts of planting structure adjustment on water saving in the Shiyang River Basin of Arid Region.
Sci Rep
December 2024
College of Geography and Environment Science, Northwest Normal University, 967 Anning East Road, Lanzhou, 730070, Gansu, China.
Planting structure adjustment (PSA) affects agricultural water saving, and is an essential part of water-saving agricultural construction. This study introduced virtual water theory and innovatively constructed a model to assess the water-saving effects of PSA in Shiyang River Basin over the past 38 years, explore the relationship between planting structure and water saving, and clarify the most water-saving planting structure. The results showed that the sown area of economic crops consistently increased as food crop areas decreased in the four counties (districts) from 1980 to 2017.
View Article and Find Full Text PDFBarley stripe mosaic virus-induced gene silencing for functional validation of abiotic stress in barley.
Funct Integr Genomics
December 2024
Department of Biology, Debre Markos University, Debre Markos, Ethiopia.
The barley stripe mosaic virus (BSMV) uses its genomic RNA components (alpha, beta, and gamma) as an efficient method for studying gene functions. It is a newly developed method that utilizes gene transcript suppression to determine the role of plant genes. BSMV derived from virus induced gene silencing (VIGS) is capable of infecting various key farming crops like barley, wheat, rice, corn, and oats.
View Article and Find Full Text PDFDetermination and Prediction of Available Energy in 13 Cereal Feed Ingredients for Growing Pigs.
Vet Sci
December 2024
State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
This study was conducted to develop equations to predict the digestible (DE) and metabolizable energy (ME) for growing pigs by using the chemical compositions of five corn, two wheat and six rice samples. A total of 13 castrated boars were chosen and fed 13 diets formulated with different cereal feed ingredients according to a 13 × 6 Youden square design. The DE and ME contents, the ratio of ME to DE, and the nutrient digestibility among the 13 cereal feed ingredients were different ( < 0.
View Article and Find Full Text PDFFunctional Characterization of , a Gene Coding an Aspartic Acid Protease in .
J Fungi (Basel)
December 2024
Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Aspartic proteases (APs), hydrolases with aspartic acid residues as catalytic active sites, are closely associated with processes such as plant growth and development and fungal and bacterial pathogenesis. is the dominant pathogenic fungus that causes Fusarium head blight (FHB) in wheat. However, the relationship of APs to the growth, development, and pathogenesis of .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!