A strictly aerobic, motile, endospore-forming, rod-shaped bacterium, designated HS21, was isolated from rhizospheric soil of the Korean fir tree (Abies koreana) from Halla mountain on Jeju island, Korea. Growth of strain HS21 was observed at pH 6.0-8.0 (optimum: pH 7.0), 0-2% (w/v) NaCl and 4-30°C (optimum: 25°C). A comparative analysis of 16S rRNA gene sequences showed that strain HS21 was most closely related to Cohnella luojiensis HY-22R (97.6%), followed by C. lupini RLAHU4B (97.4%) and C. collisoli NKM-5 (97.2%). The genome of strain HS21 comprised a circular chromosome of 7,059,027 bp with 44.8% G + C content. The DNA-DNA relatedness values between strain HS21 and C. luojiensis HY-22R and C. lupini RLAHU4B were 18.1% and 13.8%, respectively. The major cellular fatty acids (> 5%) of the isolate were anteiso-C, iso-C, C, and iso-C. The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol, and three unidentified aminophospholipids. Based on its phenotypic, phylogenetic, genomic, and chemotaxonomic properties, strain HS21 represents a novel species of the genus Cohnella, for which the name Cohnella abietis sp. nov. is proposed. The type strain is HS21 (= KCTC 43028 = CCTCC AB 2019010).
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