In this paper, an enzyme-linked immunosorbent assay (ELISA) using gold nanoparticles/metal porphyrin porous compound (Au NPs@PAF-40-Fe) as a marker was developed. Quantitative detection of C-reactive protein (CRP) was achieved by UV-visible spectrophotometry under optimal conditions. The sensor has a good linear relationship in the range of 0.05-2000 ng/mL. The lower limit of detection was 0.017 ng/mL (S/N = 3), the linear correlation coefficient was 0.9921, and the average recovery was 100.74%. The ELISA has good selectivity and provides a sensitive way for detection of CRP.
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