The specificity of fructooligosaccharides as prebiotics depends on their size and structure, which in turn depend on their origin or the synthesis procedure. In this work we describe the application of an inulosucrase (IslA) from CW28 to produce high molecular weight inulin from sucrose alongside a commercial endoinulinase (Novozym 960) produced by for a simultaneous or sequential reaction to synthesize fructooligosaccharides (FOS). The simultaneous reaction resulted in a higher substrate conversion and a wide diversity of FOS when compared to the sequential reaction. A shotgun MS analysis of the commercial endoinulinase preparation surprisingly revealed an additional enzymatic activity: a fructosyltransferase, responsible for the synthesis of FOS from sucrose. Consequentially, the range of FOS obtained in reactions combining inulosucrase from with the fructosyltransferase and endoinulinase from with sucrose as substrate may be extended and regulated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.9b03782 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics.
Biotechnol Adv
December 2021
Department of Biotechnology, Dong-A University, Busan, South Korea. Electronic address:
The current commercial plastic manufactures have been produced using petroleum-based resource. However, due to concerns over the resource depletion and the environmental sustainability, bioresource-based manufacturing processes have been developed to cope against these concerns. Bioresource-derived 2,5-furandicarboxylic acid (FDCA) can be utilized as a building block material for plastic manufactures.
View Article and Find Full Text PDFA Novel Inulin-Mediated Ethanol Precipitation Method for Separating Endo-Inulinase From Inulinases for Inulooligosaccharides Production From Inulin.
Front Bioeng Biotechnol
April 2021
William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, United States.
Inulin is a kind of polysaccharide that can be obtained various biomass. Inulooligosaccharides (IOS), a kind of oligosaccharides that can be obtained from inulin by enzymatic hydrolysis using inulinases, have been regarded as the functional food ingredients. Commercially available inulinases produced by natural contained both endo- and exo-inulinase activities.
View Article and Find Full Text PDFInvestigating the effect of different inulin-rich substrate preparations from Jerusalem artichoke ( L.) tubers on efficient inulooligosaccharides production.
Prep Biochem Biotechnol
July 2021
Department of Process Engineering, Stellenbosch University, Stellenbosch, South Africa.
Commercial production of inulooligosaccharides (IOS) relies largely on chicory roots. However, Jerusalem artichoke (JA) tubers provide a suitable alternative due to their high inulin content and low cultivation requirements. In this study, three inulin-rich substrate preparations from JA were investigated to maximize IOS production, namely powder from dried JA tuber slices (Substrate 1), solid residues after extracting protein from the JA powder (Substrate 2) and an inulin-rich fraction extracted from protein extraction residues (Substrate 3).
View Article and Find Full Text PDFEnzymatic Process Yielding a Diversity of Inulin-Type Microbial Fructooligosaccharides.
J Agric Food Chem
September 2019
Departamento de Ingeniería celular y Biocatálisis , Instituto de Biotecnología, UNAM , Avenida Universidad 2001, Colonia Chamilpa , 62420 Cuernavaca , México.
The specificity of fructooligosaccharides as prebiotics depends on their size and structure, which in turn depend on their origin or the synthesis procedure. In this work we describe the application of an inulosucrase (IslA) from CW28 to produce high molecular weight inulin from sucrose alongside a commercial endoinulinase (Novozym 960) produced by for a simultaneous or sequential reaction to synthesize fructooligosaccharides (FOS). The simultaneous reaction resulted in a higher substrate conversion and a wide diversity of FOS when compared to the sequential reaction.
View Article and Find Full Text PDFEnzymatic analysis of levan produced by lactic acid bacteria in fermented doughs.
Carbohydr Polym
March 2019
Department of Food and Nutrition, P.O. Box 27, FI-00014, University of Helsinki, Finland. Electronic address:
Levans and inulins are fructans with mainly β-(2→6) and β-(2→1) linkages, respectively. Levans are produced by many lactic acid bacteria, e.g.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!