Nitrous oxide (NO) is one of the gases with the greatest impact in the atmosphere due to its persistence and significant contribution to the greenhouse effect. This study provides an insight into the dynamics of NO production in wastewater nitrogen removal systems. A 10 L sequencing batch reactor containing enriched anammox biomass was subjected to different operational conditions, i.e., temperature, feed time, NO /NH ratio and the initial concentrations of NH and NO . Tests showed no significant differences in maximum NO production when the system was operated with a shorter feed time and no increase in the operating temperature. A higher NO production was observed when the initial NO /NH ratio increased from 1.3 to 1.7 and 1.9. The highest initial concentration of NO was linked to an increase in residual NO at the end of the batch cycle, probably due to heterotrophic denitrifying metabolism.
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http://dx.doi.org/10.2166/wst.2019.237 | DOI Listing |
Appl Microbiol Biotechnol
January 2025
Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Av. Ferrocarril San Rafael Atlixco 186, Col. Leyes de Reforma 1A Sección, Iztapalapa, CDMX, Mexico City, Mexico.
The relationship of microbial community and cometabolic consumption of 2-chlorophenol (2-CP) in a nitrifying sequencing batch reactor (SBR) was studied. The assessment of the population dynamics of the nitrifying sludge during the cometabolic 2-CP consumption with increasing ammonium (NH) concentrations in the SBR showed the presence of 39 different species of which 10 were always present in all cycles. Fifty-five percent of the species found were grouped as Proteobacteria (45% as β-proteobacteria and 10% as γ-proteobacteria class), 30% as Acidobacteria, and 15% as Deinococcus-Thermus phyla.
View Article and Find Full Text PDFBrief Bioinform
November 2024
School of Engineering, Westlake University, No. 600 Dunyu Road, 310030 Zhejiang, P.R. China.
Single-cell RNA sequencing (scRNA-seq) offers remarkable insights into cellular development and differentiation by capturing the gene expression profiles of individual cells. The role of dimensionality reduction and visualization in the interpretation of scRNA-seq data has gained widely acceptance. However, current methods face several challenges, including incomplete structure-preserving strategies and high distortion in embeddings, which fail to effectively model complex cell trajectories with multiple branches.
View Article and Find Full Text PDFBMC Biol
January 2025
Research Office, City University of Hong Kong (Dongguan), Dongguan, 523000, China.
Background: Recent advancements in single-cell RNA sequencing have greatly expanded our knowledge of the heterogeneous nature of tissues. However, robust and accurate cell type annotation continues to be a major challenge, hindered by issues such as marker specificity, batch effects, and a lack of comprehensive spatial and interaction data. Traditional annotation methods often fail to adequately address the complexity of cellular interactions and gene regulatory networks.
View Article and Find Full Text PDFWaste Manag
January 2025
Luzhou LaoJiao Co., Ltd, Luzhou 646699 China.
Solid-state fermentation of lignocellulosic waste to produce feed protein is a means of realising solid waste. However, low efficiency and susceptibility to microbial contamination remain significant challenges in feed protein production through room-temperature solid-state fermentation. In this study, thermophilic microbiomes were enriched.
View Article and Find Full Text PDFJ Environ Manage
January 2025
Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu, 610041, PR China; Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu, 610041, PR China. Electronic address:
To solve the problem of low chemical oxygen demand (COD)/N and poor efficiency of single-stage sequencing batch reactor (SBR) or anoxic/oxic process (A/O) in treatment of digested effluent of swine wastewater, combined SBR-A/O and A/O-SBR processes were employed in the addition ratios of 0, 10%, 30%, and 40% (V/V)) of raw swine wastewater (RS). Analysis of pollutants removal performance of SBR-A/O and A/O-SBR systems showed no significant difference between the two systems without RS addition. However, after adding RS, the pollutants removal efficiency of the two systems increased with the increase in the ratio of RS, with SBR-A/O system presenting better pollutants removal performance than A/O-SBR system.
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