Effect of thermal preconditioning on Hsp70 expression in the medulla oblongata and on hemodynamics during passive hyperthermia.

A short-term episode of elevated core body temperature that induces Hsp70 expression (thermal preconditioning) may protect against heatstroke during subsequent hyperthermia. The protective effects of thermal preconditioning may involve several cellular and immunological mechanisms and improvements in baroreflex sensitivity. To substantiate the hypothesis that the protective effect of thermal preconditioning also occurs in conditions with intact thermoregulation, we examined the evolution of spontaneous cardiovagal baroreflex sensitivity and the protective effect of Hsp70 expression after thermal preconditioning in nonanesthetized Wistar-Kyoto rats with implanted telemetric transmitters. In the baroreflex centers of the medulla oblongata, thermal preconditioning induced Hsp70 in perineuronal and perivascular oligodendrocytes, microglia, and endothelial cells but not in neurons. The maximal Hsp70 expression was detected 4 h after preconditioning, but a significant number of Hsp70-positive cells was still present 72 h after preconditioning. Increased c-Fos expression in the neurons of baroreflex centers was detectable only 4 h after preconditioning. The mean values of cardiovagal baroreflex sensitivity did not show significant differences during the 72-hour follow-up period after thermal preconditioning. Similarly, cardiovascular variability measures of the autonomic nervous system activity were also not significantly affected by thermal preconditioning. During passive hyperthermia, thermal preconditioning had no statistically significant effect on thermoregulation and the onset of arterial pressure decline. Our data suggest that thermal preconditioning induces a glial type of Hsp70 expression in the baroreflex centers of the medulla oblongata. However, this response was not associated with cardiovagal baroreflex sensitization and protection against hemodynamic instability during passive hyperthermia.