AI Article Synopsis
- The study presents a proteomic method to identify transcription factors that bind to specific promoters.
- The approach was validated by examining proteins that interact with the rpoS promoter of Vibrio cholerae based on cell density.
- Key proteins found in the analysis included Fis, a nucleoid-associated protein, and HapR, which regulates quorum sensing.
Article Abstract
We describe a proteomic approach to identify transcription factors binding to a target promoter. The method's usefulness was tested by identifying proteins binding to the Vibrio cholerae rpoS promoter in response to cell density. Proteins identified in this screen included the nucleoid-associated protein Fis and the quorum sensing regulator HapR.
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| http://dx.doi.org/10.1016/j.mimet.2019.105702 | DOI Listing |
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