Objective: Emergence of colistin-resistant (CREC) has generated a sense of public alarm. The objective of this study was to detect the CREC and identification of the gene responsible for such resistance.
Materials And Methods: A total of 150 samples comprising poultry cloacal swab, house flies (), and pond water were collected randomly from Mymensingh, Bangladesh and analyzed. Isolation and identification of were done based on culture and 16S rRNA gene-specific polymerase chain reaction (PCR). Phenotypic detection of CREC was done by disk diffusion method. Finally, colistin resistance genes were detected by PCR by using colistin resistant gene specific primers.
Results: Among the 150 samples, phenotypically 18.00% ( = 27/150) isolates were found as colistin resistant. By PCR, 8.00% of the isolates were found positive for the presence of gene.
Conclusions: Colistin resistant carrying are detected in poultry, house flies and water that are of great public health concern.
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| http://dx.doi.org/10.5455/javar.2019.f311 | DOI Listing |
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