Collagen type I fingerprinting (ZooMS) has recently been used to provide either palaeoenvironmental data or to identify additional hominin specimens in Pleistocene contexts, where faunal assemblages are normally highly fragmented. However, its potential to elucidate hominin subsistence behaviour has been unexplored. Here, ZooMS and zooarchaeology have been employed in a complementary approach to investigate bone assemblages from Final Mousterian and Uluzzian contexts at Fumane cave (Italy). Both approaches produced analogous species composition, but differ significantly in species abundance, particularly highlighted by a six fold-increase in the quantity of Bos/Bison remains in the molecularly identified component. Traditional zooarchaeological methods would therefore underestimate the proportion of Bos/Bison in these levels to a considerable extent. We suggest that this difference is potentially due to percussion-based carcass fragmentation of large Bos/Bison bone diaphyses. Finally, our data demonstrates high variability in species assignment to body size classes based on bone cortical thickness and fragment size. Thus, combining biomolecular and traditional zooarchaeological methods allows us to refine our understanding of bone assemblage composition associated with hominin occupation at Fumane.
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http://dx.doi.org/10.1038/s41598-019-48706-z | DOI Listing |
Sci Rep
November 2024
School of Archaeology and Museology, Peking University, Beijing, P.R. China.
PLoS One
November 2024
Department of Anthropology, University of Wyoming, Laramie, Wyoming, United States of America.
PLoS One
November 2024
Department of Archaeology and Cultural History, NTNU Vitenskapsmuseet, Norwegian University of Science and Technology, Trondheim, Norway.
PLoS One
July 2024
Departamento de Arqueología y Antropología, Institución Milá y Fontanals de Estudios en Humanidades (IMF), del Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Barcelona, Spain.
Few studies have combined the analysis of use-wear traces, traceology, and the proteomic taxonomic identification method Zooarchaeology by Mass Spectrometry (ZooMS). Traceology provides information on the usage, in this case, of bone artefacts, while ZooMS allows for taxonomic identifications where diagnostic features are otherwise gone. The approaches therefore offer complementary information on bone artefacts, allowing for insights into species selection strategies in bone tool manufacture and their subsequent use.
View Article and Find Full Text PDFBioinformatics
March 2024
Department of Evolutionary Anthropology, University of Vienna, University Biology Building, A-1030 Vienna, Austria.
Motivation: Zooarchaeology by Mass Spectrometry (ZooMS) is a palaeoproteomics method for the taxonomic determination of collagen, which traditionally involves challenging manual spectra analysis with limitations in quantitative results. As the ZooMS reference database expands, a faster and reproducible identification tool is necessary. Here we present SpecieScan, an open-access algorithm for automating taxa identification from raw MALDI-ToF mass spectrometry (MS) data.
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