Novel sonochemical synthesis of FeO nanospheres decorated on highly active reduced graphene oxide nanosheets for sensitive detection of uric acid in biological samples.

In this study, a super-active Iron (II, III) oxide nanospheres (FeO NPs) decorated reduced graphene oxide (rGOS) nanocomposite was developed. FeO NPs were stabilized on rGOS through electrostatic interactions in the aqueous medium. This process involves an ultrasound assisted reduction reaction of the GOS. The as-synthesized FeO NPs@rGOS was characterized through the HRTEM, SEM, XRD, Raman, elemental mapping and EDX analysis. The FeO NPs@rGOS modified GCE was developed for the determination of biomarker. Uric acid is important biomarker based on gout and kidney stone with high adverse effect in human body. The results obtained showed that the modified electrode FeO NPs@rGOS shows good electrochemical reduction peak compared to bare electrode and control electrodes. The FeO NPs@rGOS modified sensor linear range 0.02-783.6 µM was observed with nanomolar LOD 0.12 nM. In addition, the modified FeO NPs@rGOS/GCE sensor has been applied to determination of uric acid concentration in urine and blood serum samples. Furthermore, advantages of the modified sensor are high stability, repeatability and reproducibility.