Ethnopharmacological Relevance: Traditional Indian medicine has utilized Aeglemarmelos (L.) Corr. commonly called as bael in several indigenous systems against various diseases. Bioactive components isolated from various plant parts of A. marmelos were used in ethno-medicine. More precisely they are known for its antiviral property against various human and animal viruses.
Aim Of The Study: The study was conducted to investigate the antiviral activity of A.marmelos against Bombyx mori nucleopolyhedrovirus (BmNPV).
Materials And Methods: Among the various crude extracts tested, hexane extracts of leaves of A. marmelos with promising anti-BmNPV activity was subjected to bioactivity guided fractionation based on column chromatography. Out of 40 fractions obtained from the fractionation, fractions showing similar TLC profiles were pooled into 14 fractions. A fraction with potential activity was used to purify a molecule with anti-BmNPV activity. This molecule was characterized through structural and functional analyses.
Results: The functionally and structurally characterized molecule in the fraction with prospective anti-BmNPV activity revealed a single crystal compound 'seselin' (8, 8-dimethyl pyrido oxazine-2-one).
Conclusion: It is therefore understood that this seselin compound could be used as a natural medicine for the management of NPV infection in the silkworm larvae under commercial conditions after suitable field evaluations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jep.2019.112155 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel digestive protease chymotrypsin-like serine contributes to anti-BmNPV activity in silkworm (Bombyxmori).
Dev Comp Immunol
December 2024
Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China; Anhui International Joint Research and Developmental Center of Sericulture Resources Utilization, Hefei, 230036, China. Electronic address:
Serine proteases (SPs) are important proteases in the digestive system of lepidopteran insects. They play important roles in protein digestion, coagulation, signal transduction, hormone activation, inflammation and development. Blood-borne pyosis caused by Bombyx mori nuclear polyhedrosis virus (BmNPV) has caused serious harm to sericulture.
View Article and Find Full Text PDFRas3 in Bombyx mori with antiviral function against B. mori nucleopolyhedrovirus.
Dev Comp Immunol
March 2024
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, Southwest University, Chongqing, 400715, China.
Bombyx mori ras protein3 (BmRas3) is a small molecular protein in the GTPase superfamily, which has the activity of binding guanosine nucleotides and GTP enzymes. It acts as a molecular switch by coupling extracellular signal to different cellular response through the conversion between Ras-GTP conformation and Ras-GDP conformation, thus regulating signal pathways responsible for cell growth, migration, adhesion, survival and differentiation. However, few studies have been done on Ras3 in silkworm, and its function and mechanism are unclear.
View Article and Find Full Text PDFThe digestive proteinase trypsin, alkaline A contributes to anti-BmNPV activity in silkworm (Bombyx mori).
Dev Comp Immunol
June 2021
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China; Anhui International Joint Research and Developmental Center of Sericulture Resources Utilization, Hefei, 230036, China. Electronic address:
Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious pathogenic microorganism that causes tremendous loss to sericulture. Previous studies have found that some proteins of serine protease family in the digestive juice of B. mori larvae have anti-BmNPV activity.
View Article and Find Full Text PDFis Involved in the Response against BmNPV Infection by the Mitochondrial Apoptosis Pathway.
Insects
September 2020
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Discovery of the anti-BmNPV ( nuclearpolyhedrovirus) silkworm strain suggests that some kind of antiviral molecular mechanism does exist but is still unclear. Apoptosis, as an innate part of the immune system, plays an important role in the response against pathogen infections and may be involved in the anti-BmNPV infection. Several candidate genes involved in the mitochondrial apoptosis pathway were identified from our previous study.
View Article and Find Full Text PDFA Novel Digestive Proteinase Lipase Member H-A in Contributes to Digestive Juice Antiviral Activity Against Nucleopolyhedrovirus.
Insects
March 2020
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
Previous studies have revealed that some proteins in larvae digestive juice show antiviral activity. Here, based on the label-free proteomics data, BmLipase member H-A (BmLHA) was identified as being involved in the response to BmNPV infection in larvae digestive juice. In the present study, a gene encoding the BmLHA protein in was characterized.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!