Hydrogen peroxide (HO) plays a persuasive role in the human cell physiology. Developing an efficient assay platform and a highly sensitive tracking and quantification of HO in a physiological system is crucial to understand the neoplastic changes and/or redox homeostasis of cells. In this study, a novel turn-on latent electrochemical redox probe coupled with electrocatalytic signal amplification strategy is proposed. A custom-made readily available turn-on latent electrochemical probe 4-methoxyphenylboronic acid pinacol ester (4-MPBP) have been designed for the selective detection of endogenous HO in live cells. The electrochemical probe composed of a latent electrochemical reporter (4-methoxy phenol, 4-MP) bearing a recognition unit (boronic acid pinacol ester) for HO sensing. The selective analyte-triggered chemical transformation releases free electrochemical reporter 4-MP. The amount of HO was evaluated electrochemically at glassy carbon electrode (GCE) with a broad detection range of 0.5 μM-1 mM. An amplified signal response of released 4-MP to build a highly sensitive assay tool has been achieved via replacing the GCE transducer electrode with polydopamine@carbonnanotube-molebtinumdisulfie hybrid modified GCE as it delivered an exceptional dynamic detection range of 0.01-100 μM. The innovative blend of electrochemical molecular probe strategy, with electrocatalytic signal amplification technique has delivered outstanding assay performance at trace level sensing of HO. Next, we set up a platform for real-time in vivo monitoring of the endogenously produced HO in Caco-2 and MCF-7 cells through spermine-polyamine analogue and phorbol 12-myristate 13-acetate induction in SSAT/PAO gene and protein kinase C, respectively. As expected, the 4-MPBP latent probe coupled with electrocatalytic signal amplification strategy delivered outstanding performance for in situ HO release and tracking over time.
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http://dx.doi.org/10.1021/acssensors.9b01049 | DOI Listing |
Molecules
December 2024
Institute of Chemistry, Faculty of Science and Technology, University of Silesia, Szkolna 9, 40-007 Katowice, Poland.
A group of bithiophenyl compounds comprising the cyanoacrylate moiety were designed and successfully synthesized. The optical, (spectro)electrochemical, and aggregation-induced emission properties were studied. DFT calculations were used to explain the reaction's regioselectivity and to determine the molecules' energy parameters (i.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
December 2024
State Key Laboratory of Reliability and Intelligence of Electrical Equipment, School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300401, China.
Low-concentration electrolytes (LCEs) have attracted great attention due to their cost effectiveness and low viscosity, but suffer undesired organic-rich interfacial chemistry and poor oxidative stability. Herein, a unique latent solvent, 1,2-dibutoxyethane (DBE), is proposed to manipulate the anion-reinforced solvation sheath and construct a robust inorganic-rich interface in a 0.5 M electrolyte.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
January 2025
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, China.
Interhalogen-involved aqueous Zn||halogen batteries (AZHBs) are latent high-energy systems for grid-level energy storage, yet usually suffer from poor high-rate endurability caused by the formation of "dead species". Herein, via an electrochemically-densified cathode-interface-reaction layer (CIRL), Zn||I-Br batteries involving interhalogen reactions between the I cathode and Br from the electrolytes are initially achieved with excellent high-rate endurability. Different from that in diluted electrolytes, the CIRL formed in Br-concentrated electrolyte is denser and water-lean, which enables halogen species conversion with a more rapid charge transfer and lower activation energy.
View Article and Find Full Text PDFTalanta
February 2025
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Cancer is currently one of the major causes of human mortality and has received widespread attention. In this paper, Au@CuS composite nanomaterial as a sandwich immunosensor tag for carcinoembryonic antigen (CEA) detection strategy was studied. Herein, Au@CuS composite nanomaterials were obtained by Au nanoparticles modified with CuS, which were combined with secondary antibody (Ab) to construct an immunosensor that interacted with HO to produce a current response.
View Article and Find Full Text PDFLuminescence
October 2024
Department of Studies in Chemistry, Davangere University, Davangere, Karnataka, India.
The fluorescent materials have sparked a lot of research interests due to their unique electronic, optical and chemical characteristics. Here, we are intended to present a simple and facile synthesis of novel orange emitting thiazole-pyridone fluorescent tag (TPFT) by a simple diazo coupling reaction and the structural elucidation was carried out by IR, NMR (H and C), UV-Vis, photoluminescence and HR-MS spectrometry. The solvatochromic behaviour of the TPFT offered crucial information about the formation of hydrazone and azo tautomeric forms.
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