Development of a Molecular Marker Linked to the Locus and the Structure of HD Genes in .

Allelic differences in and mating-type loci are a prerequisite for the progression of mating in the genus ; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An -linked sequence was identified and used to find the adjacent genomic region with the entire motif of the locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker 7-2 distinguished mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the locus had a general feature consisting of the putative and genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the breeding program, and the locus could be the basis for a multi-allele detection marker.