Ectoine, the most prominent osmolyte in nature, is a vital compatible solute present in halophilic bacterium. It protects the cellular biomolecules of the halophilic bacteria and retains their intrinsic function from extreme circumstances. In the current research, ectoine biosynthesis gene cluster (ectABC) in Bacillus clausii NIOT-DSB04 was expressed heterologically in E. coli M15 (pREP4). RP-HPLC resolved several fractions of the purified recombinant product, one of which had been confirmed as ectoine. The recombinant ectoine was further characterized by H and C Nuclear Magnetic Resonance. The purified recombinant ectoine was also authenticated by FT-IR studies with the existence of ester carbonyl and C-H group. In IPTG induced E. coli M15 transgenic cells, the enzymatic activity of the ectA, B and C genes were found to be higher than that of uninduced cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.micpath.2019.103693 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metabolic engineering of Bacillus licheniformis DW2 for ectoine production.
World J Microbiol Biotechnol
January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, 368 Youyi Avenue, Wuchang District, Wuhan, Hubei, 430062, P.R. China.
Ectoine is a high-value protective agent with extensive applications in the fields of fine chemicals and biopharmaceuticals, and it is naturally synthesized by Halomonas in extreme environment, however, the current production level cannot meet the growing market demand. In this study, we aimed to develop an efficient and environmentally friendly ectoine production process using Bacillus licheniformis as the host organism. Through introducing ectoine synthetase gene cluster ectABC from Halomonas elongate, as well as optimizing ectABC expression by promoter and 5'-UTR optimization, ectoine titer was increased to 0.
View Article and Find Full Text PDFIntracellular and Extracellular Metabolic Response of the Lactic Acid Bacterium Under Salt Stress.
Metabolites
December 2024
School of Food Science and Engineering, Foshan University, Foshan 528231, China.
Background: is a member of the lactic acid bacterium group commonly found in many salt-fermented foods. Strains of isolated from high-salinity environments have been shown to tolerate salt stress to some extent. However, the specific responses and mechanisms of under salt stress are not fully understood.
View Article and Find Full Text PDFStrain W from Estuarine Sediments Dechlorinates 1,2-Dichloroethane under Elevated Salinity.
Environ Sci Technol
December 2024
Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China.
Organohalide-respiring bacteria (OHRB) have been found in various environments and play an indispensable role in the biogeochemical cycling and detoxification of halogenated organic compounds (HOCs). Currently, few ORHB have been reported to perform reductive dechlorination under high salinity conditions, indicating a knowledge gap on the diversity of OHRB and the survival strategy of OHRB in saline environments (e.g.
View Article and Find Full Text PDFEvaluation of the influence of the gas residence time and biomass concentration on methane bioconversion to ectoines in a novel Taylor flow bioreactor.
J Environ Manage
December 2024
Institute of Sustainable Processes, University of Valladolid, Dr. Mergelina s/n., 47011, Valladolid, Spain; Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina s/n., 47011, Valladolid, Spain. Electronic address:
Today, the use of biogas to produce more sustainable bioproducts is attracting an increasing attention in the quest for a circular economy. This work aims at optimizing the biosynthesis of high value bioproducts such as ectoine and hydroxyectoine from methane using a high mass transfer Taylor flow reactor and a methanotrophic consortium. The influence of the gas residence time (30-240 min) and concentration of microorganisms (0.
View Article and Find Full Text PDFFullerenol C(OH) Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2.
Int J Nanomedicine
November 2024
Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland.
Introduction And Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the nasal cavity, penetrates the nasal epithelial cells through the interaction of its spike protein with the host cell receptor angiotensin-converting enzyme 2 (ACE2) and then triggers a cytokine storm. We aimed to assess the biocompatibility of fullerenol nanoparticles C(OH) and ectoine, and to document their effect on the protection of primary human nasal epithelial cells (HNEpCs) against the effects of interaction with the fragment of virus - spike protein. This preliminary research is the first step towards the construction of a intranasal medical device with a protective, mechanical function against SARS-CoV-2 similar to that of personal protective equipment (eg masks).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!