G-quadruplex structures have been suggested to be biologically important in processes such as transcription and translation, gene expression and regulation in human cancer cells, and regulation of telomere length. Investigation of G-quadruplex structures associated with biological events is therefore essential to understanding the functions of these molecules. We developed the F-labeled nucleobases and introduced them into DNA sequences for the F NMR spectroscopy analysis. We present the F NMR methodology used in our research group for the study of G-quadruplex structures in vitro and in living cells.
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