AI Article Synopsis
- * Researchers can characterize these G-quadruplex formations using techniques like native PEG polyacrylamide gel electrophoresis, DMS footprinting, and ligand-induced photocleavage footprinting.
- * Distinctions between G-quadruplex types can be made during transcription by using specific nucleotides, such as 7-deaza-GTP or 4-thio-uridine-5'-triphosphate,
Article Abstract
In vitro transcription of double-stranded DNA (dsDNA) induces a formation of two types of G-quadruplexes, intramolecular DNA G-quadruplexes or DNA:RNA hybrid G-quadruplexes, in clusters of guanine tracts. The formation of G-quadruplexes can be characterized by native PEG polyacrylamide gel electrophoresis, DMS footprinting, and ligand-induced photocleavage footprinting. The type of G-quadruplexes can be distinguished by transcription with 7-deaza-GTP (dzGTP) or 4-thio-uridine-5'-triphosphate (4U) to detect the participation of RNA transcript.
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| http://dx.doi.org/10.1007/978-1-4939-9666-7_14 | DOI Listing |
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