AI Article Synopsis
- Amaranth seeds are a nutritious cereal in Latin America, and this study aimed to identify bioactive peptides through enzymatic hydrolysis using alcalase and flavourzyme.
- The combined use of both enzymes in a continuous process produced hydrolysates with higher bioactive potential compared to single-enzyme processes.
- Two peptide fractions exhibited significant biological activity, acting as ACE and thrombin inhibitors, along with antioxidant properties, while sequence analysis revealed previously unreported bioactive amino acid chains.
Article Abstract
spp. is a commonly grown cereal in Latin America, known for its high protein content. The objective of this study was to separate and identify bioactive peptides found in amaranth seeds through enzymatically-assisted hydrolysis using alcalase and flavourzyme. Hydrolysis was carried out for each enzyme separately and compared to two-step continuous process where both enzymes were combined. The biological activity of the resulting three hydrolysates was analyzed, finding, in general, higher bioactive potential of the hydrolysate obtained in a continuous process (combined enzymes). Its fractions were separated by RP-HPLC, and their bioactivity was analyzed. In particular, two fractions showed the highest biological activity as ACE inhibitors with IC50 at 0.158 and 0.134, thrombin inhibitors with IC50 of 167 and 155, and antioxidants in ABTS assay with SC50 at 1.375 and 0.992 mg/L, respectively. Further sequence analysis of the bioactive peptides was carried out using MALDI-TOF, which identified amino acid chains that have not been reported as bioactive so far. Bibliographic survey allowed identification of similarities between peptides reported in amaranth and other proteins. In conclusion, amaranth proteins are a potential source of peptides with multifunctional activity.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6749583 | PMC |
| http://dx.doi.org/10.3390/molecules24173033 | DOI Listing |
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