Synthesis of silver nanoparticles with antimicrobial and anti-adherence activities against multidrug-resistant isolates from .

Objectives: The spread of multidrug-resistant pathogens poses a major health threat. Silver nanoparticles represent a new-class of antimicrobial agents. The aim of this study is the microbial synthesis of silver nanoparticles and the evaluation of their antimicrobial and antibiofilm activities.

Methods: Silver nanoparticles were synthesized using cell free supernatants of . Silver nanoparticles were characterized by particle size analysis and transmission electron microscopy (TEM), and the antimicrobial and antibiofilm activities of the synthesized silver nanoparticles were assessed.

Results: The silver nanoparticle synthesis was monitored primarily by the conversion of the pale yellow colour of the bacteria free supernatants into a dark brown colour. Silver nanoparticles had uniform spherical shape, with particle sizes ranging from 37 to 168 nm and a zeta potential of -11.7 mV. Acinetobacter silver nanoparticles were effective against multidrug-resistant and with minimal inhibitory concentrations of 3.1, 1.56 and 3.1 μg/ml, respectively. Moreover, acinetobacter silver nanoparticles significantly reduced the attachment activities of and by 66.6%, 86.5% and 75%, respectively.

Conclusion: Silver nanoparticles, synthesized from inhibited microbial growth and eradicated biofilm assembly by multidrug-resistant isolates that were derived from uropathogenic infection. These results suggested the possibility of using silver nanoparticles as effective antimicrobial and antibiofilm agents against infections caused by resistant isolates.