sp. nov., isolated from tropical forest soil.

Int J Syst Evol Microbiol

State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.

Published: November 2019

A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1, was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15-37 °C and pH 6.0-8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1 was closely related to DSM 14655 (98.5 %), followed by DSM 14656 (97.9 %) and other species and formed a stable cluster with DSM 14655, DSM 14656, JCM 19319 and s CCTCC AB 2015357. Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C and Ccyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain 18x22-1 and four closely related type strains were in the range of 79.3-82.3 % and 21.9-25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 18x22-1 (=GDMCC 1.1584=KCTC 52922).

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Source
http://dx.doi.org/10.1099/ijsem.0.003641DOI Listing

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