AI Article Synopsis
- Uncultivated bacterial symbionts from the "Entotheonella" genus are known to produce various natural products associated with their sponge hosts, along with unexplored biosynthetic gene clusters in their genomes.
- A specific type III polyketide synthase (PKS) cluster named cep has been identified as being present in all known "Entotheonella" genomes, suggesting it plays a vital role in their biosynthetic capabilities.
- The enzyme CepA, part of this cep cluster, has been characterized to facilitate phenolic lipid biosynthesis, showing particular specificity for certain substrates and generating various compounds like tri- and tetraketide pyrones.
Article Abstract
Uncultivated bacterial symbionts from the candidate genus "Entotheonella" have been shown to produce diverse natural products previously attributed to their sponge hosts. In addition to these known compounds, "Entotheonella" genomes contain rich sets of biosynthetic gene clusters that lack identified natural products. Among these is a small type III polyketide synthase (PKS) cluster, one of only three clusters present in all known "Entotheonella" genomes. This conserved "Entotheonella" PKS (cep) cluster encodes the type III PKS CepA and the putative methyltransferase CepB. Herein, the characterization of CepA as an enzyme involved in phenolic lipid biosynthesis is reported. In vitro analysis showed a specificity for alkyl starter substrates and the production of tri- and tetraketide pyrones and tetraketide resorcinols. The conserved distribution of the cep cluster suggests an important role for the phenolic lipid polyketides produced in "Entotheonella" variants.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7064976 | PMC |
| http://dx.doi.org/10.1002/cbic.201900352 | DOI Listing |
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