Concentrations of coliphages, coliforms, enterococci and fluorescent Pseudomonas were monitored in several wastewater purification steps of the treatment plant Wolfenbüttel during one year. Their number varied widely during the investigation period, but was independent of seasons. In the course of sewage treatment, including primary settling, activated sludge purification, simultaneous precipitation, trickling filters and oxidation pond, the concentration of indicators decreased gradually. The coliphages were most resistant, exhibiting only a decimal elimination value of 1.7 log10 units as compared to the bacterial indicators with elimination values ranging between 2.4 and 2.8 log10 units in the whole process. The most efficient purification step revealed to be the activated sludge procedure including simultaneous phosphate precipitation with iron hydroxides and sedimentation. On an average 1.7% of the coliphages present in raw sewage or 9.8.10.11 phages were discharged into the river Oker everyday, 0.64% remained in the sludge. Numbers of indicators in the water of the oxidation pond and those seeded into river water were continuously reduced during 3 days. Also in these laboratory experiments, the coliphages were more resistant than the bacteria, but no evidence was found to support the view that coliphages play a role in the reduction of the number of coliform bacteria. Even after addition of peptone which stimulated growth of E. coli the coliphages were inactivated more rapidly. The behaviour of coliphages during the purification process is compared with literature data about enteroviruses.
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ACS ES T Water
November 2024
Waterborne Disease Prevention Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, United States.
Irrigating fresh produce with contaminated water contributes to the burden of foodborne illness. Identifying fecal contamination of irrigation waters and characterizing fecal sources and associated environmental factors can help inform fresh produce safety and health hazard management. Using two previously collected data sets, we developed and evaluated the performance of logistic regression and conditional random forest models for predicting general and human-specific fecal contamination of ponds in southwest Georgia used for fresh produce irrigation.
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December 2024
Department of Physics, University of California San Diego, La Jolla, CA, USA.
Viral DNA packaging is a required step in the assembly of many dsDNA viruses. A molecular motor fueled by ATP hydrolysis packages the viral genome to near crystalline density inside a pre-formed prohead shell in ~5 min at room temperature in vitro. We describe procedures for measuring the packaging of single DNA molecules into single viral proheads with optical tweezers.
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Antimicrobial Resistance and Phage Biocontrol Research Group (AREPHABREG), Department of Microbiology, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, North‒West University, Private Mail Bag X2046, Mmabatho, 2735, South Africa.
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Research and Production Center "MicroMir", Nizhny Kiselny Lane 5/23 Bldg 1, 107031 Moscow, Russia.
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View Article and Find Full Text PDFScience
December 2024
Institute of Functional Biology and Genomics, CSIC & University of Salamanca, Salamanca, Spain.
Bacteriophages are the most abundant and phylogenetically diverse biological entities on Earth, yet the ecological mechanisms that sustain this extraordinary diversity remain unclear. In this study, we discovered that phage diversity consistently outstripped the diversity of their bacterial hosts under simple experimental conditions. We assembled and passaged dozens of diverse phage communities on a single, nonevolving strain of until the phage communities reached equilibrium.
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