Anti-inflammatory and cytotoxic agents from sp. SWUF09-62 fungus.

The ongoing search for anti-cancer agents from microorganisms led to the isolation of four new compounds including 6-ethyl-8-hydroxy-4-chromen-4-one (), 6-ethyl-7,8-dihydroxy-4-chromen-4-one (), (3)-3,4-dihydro-8-hydroxy-7-methoxy-3-methylisocoumarin () and (3)-3,4-dihydro-5,7,8-trihydroxy-3-methylisocoumarin (), together with eleven known compounds (-) from sp. SWUF09-62 fungus. The chemical structures were deduced from IR, 1D and 2D NMR, and MS data. The absolute configurations of and were determined by ECD experiment. Compounds and indicated possible chemo-prevention and chemo-therapeutic properties, exhibited anti-inflammatory properties by reducing nitric oxide production in LPS-stimulated RAW264.7 cells (IC = 1.57 ± 0.25 and 3.02 ± 0.27 g/mL) and cytotoxicity against HT29 cells (IC = 16.46 ± 0.48 and 97.78 ± 7.14 g/mL).